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A novel structural fucosylated chondroitin sulfate from Holothuria Mexicana and its effects on growth factors binding and anticoagulation
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2017-11-02 , DOI: 10.1016/j.carbpol.2017.10.100
Qinying Li , Chao Cai , Yaoguang Chang , Fuming Zhang , Robert J. Linhardt , Changhu Xue , Guoyun Li , Guangli Yu

Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated from sea cucumber Holothuria Mexicana (FCShm) was characterized by NMR spectra and HILIC-FTMS, which demonstrated four types of branches in FCShm. Among these, two branches were α-L-Fuc-2S4S (where Fuc is fucose and S is sulfo) and α-L-Fuc-4S linked to O-3 of glucuronic acid residues, while others were identified as α-L-Fuc-4S and α-L-Fuc-3S4S attached to O-6 of N-acetylgalactosamine residue. Furthermore, the fucosyl branches were α-1,3-linked with different degree of polymerization from 1 to 5. FCShm exhibited high affinity to fibroblast growth factor 1 and 2, growth factors involved in neovascularization. Moreover, FCShm displayed intrinsic anticoagulant activity and inhibited thrombin and factor Xa activation by antithrombin III. Our results proposed a novel structural FCS and demonstrated its favorable application prospects in anti-angiogenesis and anticoagulation.



中文翻译:

墨西哥整节藻的新型结构岩藻糖基化硫酸软骨素及其对生长因子结合和抗凝作用的影响

岩藻糖基化硫酸软骨素(FCS)是海参体壁上结构独特的糖胺聚糖,具有许多生物学特性和药理作用。利用核磁共振波谱和HILIC-FTMS对墨西哥海参全氟辛烷磺酸(FCS hm)分离得到的FCS的精制结构进行了表征,证实了FCS hm中有四种分支。其中,两个分支是α-L-Fuc-2S4S(其中Fuc是岩藻糖,S是磺基)和α-L-Fuc-4S与葡萄糖醛酸残基的O -3连接,而其他分支则被确定为α-L- Fuc-4S和α-L-Fuc-3S4S附着在N的O -6上-乙酰半乳糖胺残基。此外,岩藻糖基分支是α-1,3-连接的,具有从1到5的不同聚合度。FCShm对成纤维细胞生长因子1和2显示出高亲和力,所述生长因子参与新血管形成。此外,FCS hm显示出固有的抗凝血活性,并通过抗凝血酶III抑制了凝血酶和Xa因子的活化。我们的结果提出了一种新颖的结构性FCS,并证明了其在抗血管生成和抗凝方面的有利应用前景。

更新日期:2017-12-06
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