Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated from sea cucumber Holothuria Mexicana (FCS_hm) was characterized by NMR spectra and HILIC-FTMS, which demonstrated four types of branches in FCS_hm. Among these, two branches were α-L-Fuc-2S4S (where Fuc is fucose and S is sulfo) and α-L-Fuc-4S linked to O-3 of glucuronic acid residues, while others were identified as α-L-Fuc-4S and α-L-Fuc-3S4S attached to O-6 of N-acetylgalactosamine residue. Furthermore, the fucosyl branches were α-1,3-linked with different degree of polymerization from 1 to 5. FCS_hm exhibited high affinity to fibroblast growth factor 1 and 2, growth factors involved in neovascularization. Moreover, FCS_hm displayed intrinsic anticoagulant activity and inhibited thrombin and factor Xa activation by antithrombin III. Our results proposed a novel structural FCS and demonstrated its favorable application prospects in anti-angiogenesis and anticoagulation.

岩藻糖基化硫酸软骨素（FCS）是海参体壁上结构独特的糖胺聚糖，具有许多生物学特性和药理作用。利用核磁共振波谱和HILIC-FTMS对墨西哥海参全氟辛烷磺酸（FCS _hm）分离得到的FCS的精制结构进行了表征，证实了FCS _hm中有四种分支。其中，两个分支是α-L-Fuc-2S4S（其中Fuc是岩藻糖，S是磺基）和α-L-Fuc-4S与葡萄糖醛酸残基的O -3连接，而其他分支则被确定为α-L- Fuc-4S和α-L-Fuc-3S4S附着在N的O -6上-乙酰半乳糖胺残基。此外，岩藻糖基分支是α-1，3-连接的，具有从1到5的不同聚合度_。FCShm对成纤维细胞生长因子1和2显示出高亲和力，所述生长因子参与新血管形成。此外，FCS _hm显示出固有的抗凝血活性，并通过抗凝血酶III抑制了凝血酶和Xa因子的活化。我们的结果提出了一种新颖的结构性FCS，并证明了其在抗血管生成和抗凝方面的有利应用前景。