Triclocarban (TCC) is a well-known antibacterial agent that is frequently detected in environmental, wildlife and human samples. The potential toxicological effects and action mechanism of TCC on vertebrate development has remained unclear. In the present study, we analyzed phenotypic alterations, thyroid hormone levels, thyroid hormone responsive genes, and proteomic profiles of zebrafish embryos after exposure to a series of concentrations of TCC from 6 h post-fertilization (hpf) to 120 hpf. The most nonlethal concentration (MNLC), lethal concentration 10% (LC₁₀) and lethal concentration 50% (LC₅₀) of TCC for exposures of 96 h were 133.3 μg/L, 147.5 μg/L and 215.8 μg/L, respectively. Our results showed that exposure to TCC decreased heart rate, delayed yolk absorption and swim bladder development at MNLC and LC₁₀. Exposure to MNLC of TCC inhibited thyroid hormone and altered expression of thyroid hormone responsive genes. Furthermore, exposure to 1/20 MNLC of TCC altered expression of proteins related to binding and metabolism, skeletal muscle development and function, as well as proteins involved in nervous system development and immune response, indicating TCC has potential health risks in wildlife and humans at low concentration level.

三氯卡班（TCC）是一种众所周知的抗菌剂，经常在环境，野生生物和人类样品中被检测到。TCC对脊椎动物发育的潜在毒理作用和作用机理仍不清楚。在本研究中，我们分析了从受精后6 h（hpf）到120 hpf的一系列浓度的TCC，斑马鱼胚胎的表型改变，甲状腺激素水平，甲状腺激素反应基因和蛋白质组学特征。最高非致死浓度（MNLC），致死浓度10％（LC ₁₀）和致死浓度50％（LC ₅₀暴露96 h的TCC值分别为133.3μg/ L，147.5μg/ L和215.8μg/ L。我们的结果表明，在MNLC和LC _10下，暴露于TCC会降低心率，延迟卵黄吸收和游泳膀胱发育。TCC暴露于MNLC会抑制甲状腺激素并改变甲状腺激素反应基因的表达。此外，暴露于TCC的1/20 MNLC会改变与结合和代谢，骨骼肌发育和功能有关的蛋白质的表达，以及与神经系统发育和免疫反应有关的蛋白质的表达，这表明TCC在野生动植物和人类中具有潜在的健康风险低浓度水平。