Abstract The performance of MIP is related with the thermodynamic stability of the pre-polymerization complex. The stability of pre-polymerization complex is influenced by functional monomer, cross-linker, and porogen. These factors in conjugation with the microenvironment polarity determine the binding behaviours of MIP. Methylphosphonic acid (MPA), the ultimate degradation product and persistent environmental marker of nerve agents, was selected as target. In present work, 31 P{ 1 H}NMR was employed to optimize imprinting conditions of derivative of MPA. This approach comprised of screening of functional monomer, cross-linker, porogen and stability of pre-polymerization complex before and during polymerization and the MIP was synthesized accordingly. Sorption properties of MIP were elucidated by batch rebinding studies. The selectivity of MIP towards the template was confirmed by comparing its binding with structural analogues of phosphonate esters. These results indicate that 31 P{ 1 H}NMR is a simple and effective method for the rational design of MIP for phosphorus containing environmental contaminants.

中文翻译：

---

使用 31 P{ 1 H} NMR 对用于神经毒剂标记衍生物的分子印迹聚合物进行简单设计

摘要 MIP 的性能与预聚合配合物的热力学稳定性有关。预聚合复合物的稳定性受功能单体、交联剂和致孔剂的影响。这些与微环境极性共轭的因素决定了 MIP 的结合行为。甲基膦酸 (MPA) 是神经毒剂的最终降解产物和持久性环境标志物，被选为目标。在目前的工作中，采用 31 P{ 1 H}NMR 来优化 MPA 衍生物的印迹条件。该方法包括在聚合前和聚合过程中筛选功能单体、交联剂、致孔剂和预聚合复合物的稳定性，并相应地合成 MIP。MIP 的吸附特性通过批量再结合研究阐明。通过比较 MIP 与膦酸酯结构类似物的结合，证实了 MIP 对模板的选择性。这些结果表明 31 P{ 1 H}NMR 是一种简单有效的方法，可用于合理设计含磷环境污染物的 MIP。