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Polyhydroxyalkanoates (PHA) production by photoheterotrophic microbial consortia: Effect of culture conditions over microbial population and biopolymer yield and composition.
European Polymer Journal ( IF 5.8 ) Pub Date : 2018-01-01 , DOI: 10.1016/j.eurpolymj.2017.11.007
P. Guerra-Blanco , O. Cortes , T. Poznyak , I. Chairez , E.I. García-Peña

Abstract Three microbial consortia (C2, C4, C5) grown under photoheterotrophic conditions assimilated acetate and butyrate, as individual and mixed substrates. By controlling the culture conditions, it was possible to manipulate the microbial population composition and thus the yield of polyhydroxyalkanoates (PHA) accumulation. Under limited ammonium conditions, pH control, and a sequential two-step process, C2 and C4 produced PHA. C4 showed the highest production of 44% of the cell dry mass (CDM), close to the theoretical value calculated with a stoichiometric balance. Analysis of the confocal microscopy images confirmed the accumulated biopolymer percentages produced by each consortium, and it was in close correlation with microbial distribution and substrate consumption pattern. 1H, 13C, NMR, and MALDI-TOF spectra identified the primary structure of the obtained biopolymers as copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV). This composition allows for better mechanical properties compared to the PHB homopolymer. Microbial characterization showed a similar microbial population with different proportions for C2 and C4. The highest PHA production in C4 was associated with higher abundances of PHA producers, including Clostridium (29%), Pseudomonas (8%) and Rhodopseudomonas (5%). Both microbial consortia showed that a portion of their microbial populations were able to perform syntrophic reactions (Dysgonomonas and Clostridium).

中文翻译:

光异养微生物聚生体生产聚羟基链烷酸酯 (PHA):培养条件对微生物种群和生物聚合物产量和组成的影响。

摘要 在光异养条件下生长的三个微生物聚生体(C2、C4、C5)同化了乙酸盐和丁酸盐,作为单独的和混合的底物。通过控制培养条件,可以控制微生物种群组成,从而控制聚羟基链烷酸酯 (PHA) 积累的产量。在有限的铵条件、pH 控制和连续的两步法下,C2 和 C4 产生 PHA。C4 显示出最高的细胞干质量 (CDM) 的 44%,接近使用化学计量平衡计算的理论值。共聚焦显微镜图像的分析证实了每个联合体产生的累积生物聚合物百分比,并且与微生物分布和底物消耗模式密切相关。1H、13C、核磁共振、和 MALDI-TOF 光谱确定所获得的生物聚合物的一级结构为 3-羟基丁酸酯 (3HB) 和 3-羟基戊酸酯 (3HV) 的共聚物。与 PHB 均聚物相比,该组合物具有更好的机械性能。微生物表征表明,C2 和 C4 具有不同比例的相似微生物种群。C4 中最高的 PHA 产量与 PHA 生产者的丰度较高有关,包括梭菌 (29%)、假单胞菌 (8%) 和红假单胞菌 (5%)。两个微生物联盟都表明,它们的一部分微生物种群能够进行同养反应(Dysgonomonas 和 Clostridium)。微生物表征表明,C2 和 C4 具有不同比例的相似微生物种群。C4 中最高的 PHA 产量与 PHA 生产者的丰度较高有关,包括梭菌 (29%)、假单胞菌 (8%) 和红假单胞菌 (5%)。两个微生物联盟都表明,它们的一部分微生物种群能够进行同养反应(Dysgonomonas 和 Clostridium)。微生物表征表明,C2 和 C4 具有不同比例的相似微生物种群。C4 中最高的 PHA 产量与 PHA 生产者的丰度较高有关,包括梭菌 (29%)、假单胞菌 (8%) 和红假单胞菌 (5%)。两个微生物联盟都表明,它们的一部分微生物种群能够进行同养反应(Dysgonomonas 和 Clostridium)。
更新日期:2018-01-01
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