The development of bacteria repellent surface coatings is critical in various fields ranging from biosensing to health care, biotechnology and food production. In the present study we exploit that the protein flagellin rapidly forms a dense and oriented monolayer on hydrophobic surfaces upon adsorption from aqueous solution. This oriented layer mimics the surface of bacterial flagellar filaments and has excellent bacteria repellent properties. In situ OWLS (Optical Waveguide Lightmode Spectroscopy) measurements were used to monitor on-line both the formation of the protein layer on the silanized sensor surface and subsequent bacterial adhesion. The adhered cells were also visualized by fluorescent microscopy and the formed protein film was characterized by AFM (Atomic Force Microscopy). In parallel control experiments, the adherence of bacteria was measured on bare hydrophobic surfaces as well. Both OWLS and microscopy results well confirmed that the flagellin coating drastically reduced the adhesion of E. coli cells. Therefore, a novel type of bacteria repellent layer made of flagellin is demonstrated.

在从生物传感到医疗保健，生物技术和食品生产等各个领域，抗细菌表面涂层的开发至关重要。在本研究中，我们利用蛋白质鞭毛蛋白从水溶液中吸附后迅速在疏水表面上形成致密且定向的单层。该定向层模仿细菌鞭毛细丝的表面，并具有出色的细菌排斥特性。原位OWLS（光学波导光模光谱）测量用于在线监测硅烷化传感器表面上蛋白质层的形成以及随后的细菌粘附。还通过荧光显微镜观察粘附的细胞，并通过AFM（原子力显微镜）表征形成的蛋白膜。在平行对照实验中，还测量了细菌在裸露的疏水性表面上的附着力。OWLS和显微镜检查结果均充分证实，鞭毛蛋白涂层可大大降低大肠杆菌细胞的粘附力。因此，证明了由鞭毛蛋白制成的新型的细菌排斥层。