Viruses accomplish their replication by exploiting many cellular resources, including metabolites and energy. Similarly to other (+)RNA viruses, tomato bushy stunt virus (TBSV) induces major changes in infected cells. However, the source of energy required to fuel TBSV replication is unknown. We find that TBSV co-opts the cellular glycolytic ATP-generating pyruvate kinase (PK) directly into the viral replicase complex to boost progeny RNA synthesis. The co-opted PK generates high levels of ATP within the viral replication compartment at the expense of a reduction in cytosolic ATP pools. The ATP generated by the co-opted PK is used to promote the helicase activity of recruited cellular DEAD-box helicases, which are involved in the production of excess viral (+)RNA progeny. Altogether, recruitment of PK and local production of ATP within the replication compartment allow the virus replication machinery an access to plentiful ATP, facilitating robust virus replication.

病毒通过利用许多细胞资源（包括代谢物和能量）来完成复制。与其他 (+)RNA 病毒类似，番茄丛状矮化病毒 (TBSV) 会诱导受感染细胞发生重大变化。然而，推动 TBSV 复制所需的能量来源尚不清楚。我们发现 TBSV 将细胞糖酵解 ATP 生成丙酮酸激酶 (PK) 直接纳入病毒复制酶复合物中，以促进子代 RNA 合成。增选 PK 在病毒复制区室中产生高水平 ATP，但代价是胞质 ATP 库减少。由增选 PK 产生的 ATP 用于促进招募的细胞 DEAD-box 解旋酶的解旋酶活性，这些解旋酶参与过量病毒 (+)RNA 后代的产生。总而言之，复制室中 PK 的募集和 ATP 的本地产生使病毒复制机制能够获得充足的 ATP，从而促进病毒的稳健复制。