N⁶-Methyladenosine (m⁶A) is the most prevalent post-transcriptional modification of eukaryotic mRNA and long noncoding RNA. m⁶A mediates its effects primarily by recruiting proteins, including the multiprotein eukaryotic initiation factor 3 complex and a set of proteins that contain the YTH domain. Here we describe the mechanisms by which YTH domain-containing proteins bind m⁶A and influence the fate of m⁶A-containing RNA in mammalian cells. We discuss the diverse, and occasionally contradictory, functions ascribed to these proteins and the emerging concepts that are influencing our understanding of these proteins and their effects on the epitranscriptome.

N ^{6 -}甲基腺苷 (m ⁶ A) 是真核 mRNA 和长链非编码 RNA 最普遍的转录后修饰。m ⁶ A 主要通过募集蛋白质来调节其作用，包括多蛋白真核起始因子 3 复合物和一组包含 YTH 结构域的蛋白质。在这里，我们描述了含有 YTH 结构域的蛋白质结合 m ⁶ A 并影响哺乳动物细胞中含有m ⁶ A 的 RNA的命运的机制。我们讨论了归因于这些蛋白质的多样化，有时甚至是相互矛盾的功能，以及影响我们对这些蛋白质及其对表观转录组影响的理解的新兴概念。