The value of exploring selectivity and solvent strength ternary gradients in enhanced fluidity liquid chromatography (EFLC) is demonstrated for the separation of inulin-type fructans from chicory. Commercial binary pump systems for supercritical fluid chromatography only allow for the implementation of ternary solvent strength gradients which can be restrictive for the separation of polar polymeric analytes. In this work, a custom system was designed to extend the capability of EFLC to allow tuning of selectivity or solvent strength in ternary gradients. Gradient profiles were evaluated using the Berridge function (RF1), normalized resolution product (NRP), and gradient peak capacity (Pc). Selectivity gradients provided the separation of more analytes over time. The RF1 function showed favor to selectivity gradients with comparable Pc to that of solvent strength gradients. NRP did not strongly correlate with Pc or RF1 score. EFLC with the hydrophilic interaction chromatography, HILIC, separation mode was successfully employed to separate up to 47 fructan analytes in less than 25 min using a selectivity gradient.

中文翻译：

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使用三元溶剂强度和选择性梯度增强菊糖果聚糖的流动性液相色谱

证明了在增强流动性液相色谱 (EFLC) 中探索选择性和溶剂强度三元梯度对于从菊苣中分离菊粉型果聚糖的价值。用于超临界流体色谱的商用二元泵系统仅允许实施三元溶剂强度梯度，这可能限制极性聚合物分析物的分离。在这项工作中，设计了一个定制系统来扩展 EFLC 的能力，以允许在三元梯度中调整选择性或溶剂强度。使用 Berridge 函数 (RF1)、归一化分辨率积 (NRP) 和梯度峰容量 (Pc) 评估梯度曲线。随着时间的推移，选择性梯度提供了更多分析物的分离。RF1 函数显示出有利于具有与溶剂强度梯度相当的 Pc 的选择性梯度。NRP 与 Pc 或 RF1 评分没有强相关性。EFLC 与亲水相互作用色谱 HILIC 分离模式成功地使用选择性梯度在不到 25 分钟的时间内分离了多达 47 种果聚糖分析物。