Isomer-specific analysis of perfluoroalkyl acids (PFAAs) is important to accurately assess their environmental source, fate, and human risks. In this study, a method was developed for the determination of perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), and perfluorohexane sulfonate (PFHxS) isomers in biosolids, biosolids-amended soils and plants using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS). The separation efficiencies of two chromatographic columns and extraction capacities of different methods were tested. Compared with the C18 column (ACQUITY UPLC BEH Shield RP18 column), the column with an alkyl perfluorinated C8 stationary phase (Epic FO LB column), in combination with the distinct MS/MS transitions of analytes, allowed better separation of most isomers. The ion-pair extraction method showed more effective matrix separation than that of the alkaline digestion method, with recoveries ranging from 79.6-105% for biosolids, 80.4-116% for soils, and 68.0-114% for plant tissues. The method detection limits ranged from 10 to 55, 3–13, and 8–58 pg/g dry weight for biosolids, soil, and plants, respectively. This method was applied successfully to quantify individual isomers in biosolids, biosolids-amended soils and plants. Six PFOA, eight PFOS, and two PFHxS isomers were found in the samples, with linear isomers being the dominant species. Further analysis revealed that the translocation potentials of branched isomers within plants were higher than those of linear isomers.

全氟烷基酸（PFAA）的异构体特异性分析对于准确评估其环境来源，命运和人类风险非常重要。在这项研究中，开发了一种使用超高效液相色谱-串联质谱法测定生物固体，生物固体修饰的土壤和植物中全氟辛酸（PFOA），全氟辛烷磺酸盐（PFOS）和全氟己烷磺酸盐（PFHxS）异构体的方法。光谱法（UHPLC–MS / MS）。测试了两个色谱柱的分离效率和不同方法的提取能力。与C18色谱柱（ACQUITY UPLC BEH Shield RP18色谱柱）相比，具有烷基全氟化C8固定相的色谱柱（Epic FO LB色谱柱）与分析物独特的MS / MS转换相结合，可以更好地分离大多数异构体。离子对萃取法显示出比碱性消化法更有效的基质分离，生物固体的回收率范围为79.6-105％，土壤的回收率范围为80.4-116％，植物组织的回收率范围为68.0-114％。对于生物固体，土壤和植物，该方法的检测限分别为干重10至55、3-13和8-58 pg / g。该方法已成功应用于定量生物固体，经生物固体改良的土壤和植物中的单个异构体。在样品中发现了6种PFOA，8种PFOS和2种PFHxS异构体，其中线性异构体为主要物种。进一步的分析表明，植物中支链异构体的易位电位高于线性异构体。生物固体占6-105％，土壤占80.4-116％，植物组织占68.0-114％。对于生物固体，土壤和植物，该方法的检测限分别为干重10至55、3-13和8-58 pg / g。该方法已成功应用于定量生物固体，经生物固体改良的土壤和植物中的单个异构体。在样品中发现了6种PFOA，8种PFOS和2种PFHxS异构体，其中线性异构体为主要物种。进一步的分析表明，植物中支链异构体的易位电位高于线性异构体。生物固体占6-105％，土壤占80.4-116％，植物组织占68.0-114％。对于生物固体，土壤和植物，该方法的检测限分别为干重10至55、3-13和8-58 pg / g。该方法已成功应用于定量生物固体，经生物固体改良的土壤和植物中的单个异构体。在样品中发现了6种PFOA，8种PFOS和2种PFHxS异构体，其中线性异构体为主要物种。进一步的分析表明，植物中支链异构体的易位电位高于线性异构体。生物固体改良的土壤和植物。在样品中发现了6种PFOA，8种PFOS和2种PFHxS异构体，其中线性异构体为主要物种。进一步的分析表明，植物中支链异构体的易位电位高于线性异构体。生物固体改良的土壤和植物。在样品中发现了6种PFOA，8种PFOS和2种PFHxS异构体，其中线性异构体为主要物种。进一步的分析表明，植物中支链异构体的易位电位高于线性异构体。