Long-Term Preservation of Bacteriophage Antimicrobials Using Sugar Glasses,ACS Biomaterials Science & Engineering

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Long-Term Preservation of Bacteriophage Antimicrobials Using Sugar Glasses
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-11-09 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00468
Vincent Leung ₁ , Alexandra Szewczyk ₁ , Jacqueline Chau ₁ , Zeinab Hosseinidoust ₁ , Logan Groves ₁ , Hajar Hawsawi ₂ , Hany Anany _{2,

3} , Mansel W. Griffiths ₂ , M. Monsur Ali ₁ , Carlos D. M. Filipe ₁

Affiliation

The antimicrobial activity of LISTEX P100, Salmonella CG4, and E. coli AG10 bacteriophages were preserved in pullulan-trehalose mixture as dried films and as coatings on food packaging. The phages encapsulated in pullulan-trehalose films were able to retain infectivity for up to 3 months at ambient storage conditions. Various buffers, disaccharides and disaccharide concentrations were investigated to optimize the long-term stability of the phages in the films. It was found that pullulan and trehalose need to be simultaneously present in the film to provide the stabilizing effect and that the presence of buffers that lead to the formation of crystals in the films must be avoided for phage activity to be maintained. Overall, this study describes a method of preserving bacteriophage activity in a dried format that has great potential for use as coatings, which can be used to create antimicrobial surfaces for food preparation and for food preservation.

中文翻译：

使用糖杯长期保存噬菌体抗菌素

LISTEX P100，沙门氏菌CG4和大肠杆菌的抗菌活性将AG10噬菌体保存在支链淀粉-海藻糖混合物中，作为干膜和食品包装上的涂层。封装在支链淀粉-海藻糖膜中的噬菌体在环境储存条件下能够保持长达3个月的感染性。研究了各种缓冲液，二糖和二糖浓度，以优化膜中噬菌体的长期稳定性。发现在膜中需要同时存在支链淀粉和海藻糖以提供稳定作用，并且必须避免导致膜中形成晶体的缓冲液的存在以维持噬菌体活性。总体而言，这项研究描述了一种以干燥形式保存噬菌体活性的方法，该方法具有用作涂料的巨大潜力，

更新日期：2017-11-09

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