Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

中文翻译：

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使用基于iTRAQ和基于TMT的等压标记分析血浆蛋白质组

在过去的十年中，使用等压串联质谱标签进行化学标记，例如相对和绝对定量试剂（iTRAQ）和串联质谱标签（TMT）试剂的等压标签，已广泛用于各种临床取向的血清和血浆蛋白质组学中学习。在这篇综述中，这些工作的范围是对研究领域，所采用的方法和性能限制的关注。这些应用涵盖了广泛的疾病，病症和感染，并且已经实现了各种不同的制备和质谱方法。与难以量化数百种蛋白质的早期研究相比，越来越多的研究为血浆蛋白质组学提供了更深刻的见解，将量化蛋白质的数量扩展到了上千种。