Resonance Raman detection of antioxidants using an iron oxide nanoparticle catalysed decolourisation assay,Analyst

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Resonance Raman detection of antioxidants using an iron oxide nanoparticle catalysed decolourisation assay
Analyst ( IF 3.6 ) Pub Date : 2017-11-03 00:00:00 , DOI: 10.1039/c7an01151b
Sian Sloan-Dennison _{1,

2,

3,

4,

5} , Neil C. Shand _{5,

6,

7,

8} , Duncan Graham _{1,

2,

3,

4,

5} , Karen Faulds _{1,

2,

3,

4,

5}

Affiliation

Nanozymes are metal nanoparticles with catalytic properties that can be used to oxidise peroxidase substrates giving a colorimetric response which can be detected using UV-vis, and recently, Raman spectroscopy. Due to their ease of synthesis and increased stability, nanozymes are being increasing investigated to replace conventional enzymes for the detection of biomolecules. Here we exploit the catalytic activity of iron oxide (Fe₂O₃) nanoparticles combined with the substrate 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) in a decolourisation assay for the detection of antioxidants. Fe₂O₃ nanoparticles were used to catalyse the oxidation of ABTS to its green radical cation which, upon the addition of an antioxidant, resulted in a decolourisation due to the reduction of the radical cation caused by the hydrogen donating antioxidant. The assay was applied for the detection of multiple antioxidants (glutathione, chlorogenic acid and ascorbic acid), and was followed by monitoring the resonance Raman scattering from the ABTS solution using a portable Raman system with 785 nm laser excitation. This novel assay has the potential to be optimised to detect antioxidant activity in body fluid with low limits of detection with point of use monitoring.

中文翻译：

使用氧化铁纳米颗粒催化的脱色测定共振拉曼法检测抗氧化剂

纳米酶是具有催化性能的金属纳米颗粒，可用于氧化过氧化物酶底物，产生比色响应，可使用紫外可见和最近的拉曼光谱法进行检测。由于它们易于合成和增加的稳定性，人们正在对纳米酶进行研究，以取代用于检测生物分子的常规酶。在这里，我们利用氧化铁（Fe ₂ O ₃）纳米粒子与底物2,2-叠氮基双（3-乙基-苯并噻唑啉-6-磺酸）（ABTS）的结合在脱色测定中的催化活性来检测抗氧化剂。铁₂ O ₃纳米颗粒用于催化ABTS氧化为其绿色自由基阳离子，加入抗氧化剂后，由于供氢抗氧化剂导致自由基阳离子的减少，导致脱色。该测定法用于检测多种抗氧化剂（谷胱甘肽，绿原酸和抗坏血酸），然后使用便携式拉曼系统和785 nm激光激发，监测ABTS溶液的共振拉曼散射。这种新颖的测定方法具有优化的潜力，可通过使用点监测的低检测限来检测体液中的抗氧化活性。

更新日期：2017-11-09

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