In Vivo Oxidative Stress Monitoring Through Intracellular Hydroxyl Radicals Detection by Recyclable Upconversion Nanoprobes,Analytical Chemistry

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In Vivo Oxidative Stress Monitoring Through Intracellular Hydroxyl Radicals Detection by Recyclable Upconversion Nanoprobes
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-09 00:00:00 , DOI: 10.1021/acs.analchem.7b03270
Yuxin Liu ₁ , Qi Jia ₁ , Quanwei Guo ₁ , Anqi Jiang ₁ , Jing Zhou ₁

Affiliation

Oxidative stress, as an essential cause to many diseases via irreversible biomolecule oxidation, can be induced by casual-contacted nanomaterials, which can generate hydroxyl radical (•OH) due to their active surface capacity. Herein, we report a novel upconversion nanoprobe for ratiometric •OH detection and monitoring titanium oxide nanomaterial-induced oxidative stress. Unlike previously developed nanoprobes, the •OH-responsive acceptor in these nanoprobes can be rapidly prepared and easily recycled. With a detection limit of ∼2 nM and a broad linear range of 4 nM–16 μM, the nanoprobe is suitable for both in vitro and in vivo applications. The facile preparation and recyclable strategy provide a new method for developing novel nanoprobes that can directly detect hazardous molecules in biological samples and systems.

中文翻译：

通过可回收的上转换纳米探针通过细胞内羟自由基检测的体内氧化应激监测。

偶然接触的纳米材料可诱发氧化应激，这是通过不可逆的生物分子氧化导致许多疾病的重要原因，由于其有效的表面容量，它们会产生羟基自由基（• OH）。在这里，我们报告了一种新型的上转换纳米探针，用于比率式• OH检测和监测氧化钛纳米材料诱导的氧化应激。与以前开发的纳米探针不同，•这些纳米探针中的OH响应受体可以快速制备并易于回收利用。纳米探针的检测极限为约2 nM，线性范围为4 nM–16μM，适用于体外和体内应用。简便的制备和可循环利用策略为开发可直接检测生物样品和系统中的有害分子的新型纳米探针提供了一种新方法。

更新日期：2017-11-09

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