Geometrical Alignment of Multiple Fabrication Steps for Rapid Prototyping of Microfluidic Paper-Based Analytical Devices,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Geometrical Alignment of Multiple Fabrication Steps for Rapid Prototyping of Microfluidic Paper-Based Analytical Devices
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-09 00:00:00 , DOI: 10.1021/acs.analchem.7b03796
Mohammad Rahbar ₁ , Pavel N. Nesterenko ₁ , Brett Paull ₁ , Mirek Macka ₁

Affiliation

Three main fabrication steps for microfluidic paper-based analytical devices (μPADs) were fully integrated with accurate geometrical alignment between the individual steps in a simple and rapid manner. A wax printer for creating hydrophobic barriers was integrated with an inexpensive (ca. $300) electronic craft plotter/cutter for paper cutting, followed by colorimetric reagent deposition using technical pens. The principal shortcoming in the lack of accurate and precise alignment of the features created by these three individual fabrication steps was addressed in this work by developing appropriate alignment procedures during the multistep fabrication process. The wax printing step was geometrically aligned with the following cutting and plotting (deposition) steps in a highly accurate and precise manner using optical scanning function of the plotter/cutter based on registration marks printed on the paper using the wax printer and scanned by the plotter/cutter. The accuracy and precision of alignment in a two-dimensional plane were quantified by cutting and plotting cross-shaped features and measuring their center coordinates relative to wax printed reference lines. The average accuracy along the X- and Y-axis was 0.12 and 0.16 mm for cutting and 0.19 and 0.17 mm for plotting, respectively. The potential of this approach was demonstrated by fabricating μPADs for instrument-free determination of cobalt in waters using distance-based readout, with excellent precision (%RSD = 5.7) and detection limit (LOD) of 2.5 ng and 0.5 mg/L (mass and concentration LODs, respectively).

中文翻译：

多个制造步骤的几何对准，用于基于微流纸的分析设备快速原型化

基于微流纸的分析设备（μPAD）的三个主要制造步骤以简单，快速的方式完全集成在一起，并且各个步骤之间具有精确的几何对齐方式。将用于产生疏水性屏障的蜡印刷机与便宜的（约300美元）电子工艺绘图仪/切割机集成在一起以进行纸张切割，然后使用技术笔进行比色试剂沉积。在这项工作中，通过在多步制造过程中开发适当的对准程序，解决了由这三个单独的制造步骤所产生的特征缺乏精确和精确对准的主要缺点。基于绘图仪/切割机的光学扫描功能，基于蜡打印机打印在纸上并由绘图仪扫描的套准标记，以高精度和精确的方式将蜡的打印步骤与随后的切割和绘图（沉积）步骤进行几何对齐/刀具。通过切割和绘制十字形特征并测量其相对于蜡质打印参考线的中心坐标，可以量化二维平面中对齐的精度和精确度。沿线的平均准确度通过切割和绘制十字形特征并测量其相对于蜡质打印参考线的中心坐标，可以量化二维平面中对齐的精度和精确度。沿线的平均准确度通过切割和绘制十字形特征并测量其相对于蜡质打印参考线的中心坐标，可以量化二维平面中对齐的精度和精确度。沿线的平均准确度切割的X轴和Y轴分别为0.12和0.16毫米，标绘为0.19和0.17毫米。通过制造基于距离的读数的μPAD无需仪器即可测定水中的钴，这种方法的潜力得到了证明，具有出色的精密度（％RSD = 5.7）和2.5 ng和0.5 mg / L（质量）的检测限（LOD）和集中LOD）。

更新日期：2017-11-09

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11