Calcium isotope signature: new proxy for net change in bone volume for chronic kidney disease and diabetic rats,Metallomics

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Calcium isotope signature: new proxy for net change in bone volume for chronic kidney disease and diabetic rats
Metallomics ( IF 2.9 ) Pub Date : 2017-10-25 00:00:00 , DOI: 10.1039/c7mt00255f
Yu-ki Tanaka _{1,

2,

3,

4,

5} , Nobuyuki Yajima _{4,

5,

6,

7,

8} , Yusuke Higuchi _{4,

7,

9,

10} , Hideyuki Yamato _{4,

7,

9,

10} , Takafumi Hirata _{1,

2,

3,

4,

11}

Affiliation

Herein, we measure the Ca isotope ratios (⁴⁴Ca/⁴²Ca and ⁴³Ca/⁴²Ca) in serum and bone samples collected from rats with chronic kidney disease (CKD) or diabetes mellitus (DM). For the serum samples, the isotope ratios are lower for the CKD (δ⁴⁴Ca/⁴²Ca_serum = 0.16 ± 0.11‰; 2SD, n = 6) and the DM (δ⁴⁴Ca/⁴²Ca_serum = −0.11 ± 0.25‰; 2SD, n = 7) rats than that for the control rats (δ⁴⁴Ca/⁴²Ca_serum = 0.25 ± 0.04‰; 2SD, n = 7). Bone samples from two distinct positions of 20 rats in total, namely, the center and proximal parts of the tibial diaphysis, are subject to Ca isotope analysis. The resulting δ⁴⁴Ca/⁴²Ca values for the bone of the proximal part are about 0.3‰ lower than that for the serum samples from the same rats. The larger isotope fractionations between the serum and bone are consistent with previously reported data for vertebrate animals (e.g., Skulan and DePaolo, 1999), which suggests the preferential incorporation of lighter Ca isotopes through bone formation. For the bones from the control and CKD rats, there were no differences in the δ⁴⁴Ca/⁴²Ca values between the positions of the bone. In contrast, the δ⁴⁴Ca/⁴²Ca values of the bone for the DM rats were different between the positions of the bone. Due to the lower bone turnover rate for the DM rats, the δ⁴⁴Ca/⁴²Ca for the middle of the diaphysis can reflect the Ca isotopes in the bone formed prior to the progression of DM states. Thus, the resulting δ⁴⁴Ca/⁴²Ca values show a clear correlation with bone mineral density (BMD). This can be due to the release of isotopically lighter Ca from the bone to the serum. In the present study, our data demonstrate that the δ⁴⁴Ca/⁴²Ca value for serum can be used as a new biomarker for evaluating changes in bone turnover rate, followed by changes in bone volume.

中文翻译：

钙同位素特征：慢性肾脏疾病和糖尿病大鼠骨体积净变化的新替代物

本文中，我们测量了从患有慢性肾脏病（CKD）或糖尿病（DM）的大鼠收集的血清和骨样品中的Ca同位素比（⁴⁴ Ca / ⁴² Ca和⁴³ Ca / ⁴² Ca）。对于血清样品，所述同位素比率都为CKD下（δ ⁴⁴的Ca / ⁴²钙_血清= 0.16±0.11‰; 2SD，Ñ = 6）和DM（δ ⁴⁴的Ca / ⁴²钙_血清= -0.11±0.25‰ ; 2SD，ñ = 7）的大鼠相比，对于对照组大鼠（δ ⁴⁴的Ca / ⁴²钙_血清= 0.25±0.04‰; 2SD，n = 7）。对总共20只大鼠的两个不同位置（即胫骨干端的中部和近端）的骨骼样品进行Ca同位素分析。将得到的δ ⁴⁴的Ca / ⁴²的近端部分的骨的Ca的值是约0.3‰比用于从相同的大鼠血清样品降低。血清和骨骼之间较大的同位素分馏与先前报道的脊椎动物相关数据一致（例如Skulan和DePaolo，1999），这表明较轻的Ca同位素优先通过骨骼形成而掺入。从控制和CKD大鼠骨骼，有在无差异δ ⁴⁴的Ca / ⁴²Ca值在骨骼位置之间。与此相反，δ ⁴⁴的Ca / ⁴²的骨的糖尿病大鼠的Ca值在骨的位置之间不同。由于较低的骨周转率的DM大鼠中，δ ⁴⁴的Ca / ⁴²钙对骨干的中间可以反映在钙DM状态的进展之前形成骨同位素。因此，得到的δ ⁴⁴的Ca / ⁴²钙值显示出与骨矿物质密度（BMD）的明确的相关性。这可能是由于从骨骼到血清释放出同位素较轻的Ca。在本研究中，我们的数据表明，δ ⁴⁴的Ca / ⁴²血清中的Ca值可以用作评估骨转换率变化，然后评估骨量变化的新生物标记。

更新日期：2017-11-08

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