Ester Synthesis in Water: Mycobacterium smegmatis Acyl Transferase for Kinetic Resolutions,Advanced Synthesis & Catalysis

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Ester Synthesis in Water: Mycobacterium smegmatis Acyl Transferase for Kinetic Resolutions
Advanced Synthesis & Catalysis ( IF 4.4 ) Pub Date : 2017-11-08 06:50:29 , DOI: 10.1002/adsc.201701282
Nicolas de Leeuw ₁ , Guzman Torrelo ₁ , Carolin Bisterfeld ₁ , Verena Resch ₁ , Luuk Mestrom ₁ , Emanuele Straulino ₁ , Laura van der Weel ₁ , Ulf Hanefeld ₁

Affiliation

The acyl transferase from Mycobacterium smegmatis (MsAcT) catalyses transesterification reactions in aqueous media because of its hydrophobic active site. Aliphatic cyanohydrin and alkyne esters can be synthesised in water with excellent and strikingly opposite enantioselectivity [(R);E>37 and (S);E>100, respectively]. When using this enzyme, the undesired hydrolysis of the acyl donor is an important factor to take into account. Finally, the choice of acyl donor can significantly influence the obtained enantiomeric excesses.

中文翻译：

水中的酯合成：耻垢分枝杆菌酰基转移酶的动力学分辨率

耻垢分枝杆菌（MsAcT）的酰基转移酶由于其疏水性活性位点而在水性介质中催化酯交换反应。脂肪族氰醇和炔基酯可以在水中合成，其对映选择性极好且非常相反[分别为[（R）; E> 37和（S）; E> 100]]。当使用这种酶时，酰基供体的不希望的水解是要考虑的重要因素。最后，酰基供体的选择可以显着影响获得的对映体过量。

更新日期：2017-11-08

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