A T7 RNA Polymerase Mutant Enhances the Yield of 5′‐Thienoguanosine‐Initiated RNAs,ChemBioChem

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A T7 RNA Polymerase Mutant Enhances the Yield of 5′‐Thienoguanosine‐Initiated RNAs
ChemBioChem ( IF 2.6 ) Pub Date : 2017-12-07 , DOI: 10.1002/cbic.201700538
Seth Lyon ₁ , Venkat Gopalan ₁

Affiliation

Improved 5′‐modification of RNA: Premature termination of RNAs initiated with guanosine analogues has rendered it difficult to introduce 5′‐guanosine modifications into RNAs generated by in vitro transcription with T7 RNA polymerase (T7RNAP). Here, we provide a solution by using a mutant T7RNAP with a decreased propensity for abortive transcription.

中文翻译：

T7 RNA聚合酶突变体提高了5'-硫鸟嘌呤引发的RNA的产量

改进了RNA的5'-修饰：鸟嘌呤类似物引发的RNA的过早终止使得难以将5'-鸟苷修饰引入通过T7 RNA聚合酶（T7RNAP）体外转录产生的RNA中。在这里，我们提供了通过使用突变T7RNAP降低流产转录倾向的解决方案。

更新日期：2017-12-07

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