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Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation
ACS Omega ( IF 3.7 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acsomega.7b01303 Vladimíra Moulisová 1 , Sara Poveda-Reyes 2 , Esther Sanmartín-Masiá 2 , Luis Quintanilla-Sierra 3, 4 , Manuel Salmerón-Sánchez 1 , Gloria Gallego Ferrer 2, 4
ACS Omega ( IF 3.7 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acsomega.7b01303 Vladimíra Moulisová 1 , Sara Poveda-Reyes 2 , Esther Sanmartín-Masiá 2 , Luis Quintanilla-Sierra 3, 4 , Manuel Salmerón-Sánchez 1 , Gloria Gallego Ferrer 2, 4
Affiliation
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Gelatin–hyaluronic acid (Gel–HA) hybrid hydrogels have been proposed as matrices for tissue engineering because of their ability to mimic the architecture of the extracellular matrix. Our aim was to explore whether tyramine conjugates of Gel and HA, producing injectable hydrogels, are able to induce a particular phenotype of encapsulated human mesenchymal stem cells without the need for growth factors. While pure Gel allowed good cell adhesion without remarkable differentiation and pure HA triggered chondrogenic differentiation without cell spreading, the hybrids, especially those rich in HA, promoted chondrogenic differentiation as well as cell proliferation and adhesion. Secretion of chondrogenic markers such as aggrecan, SOX-9, collagen type II, and glycosaminoglycans was observed, whereas osteogenic, myogenic, and adipogenic markers (RUNX2, sarcomeric myosin, and lipoproteinlipase, respectively) were not present after 2 weeks in the growth medium. The most promising matrix for chondrogenesis seems to be a mixture containing 70% HA and 30% Gel as it is the material with the best mechanical properties from all compositions tested here, and at the same time, it provides an environment suitable for balanced cell adhesion and chondrogenic differentiation. Thus, it represents a system that has a high potential to be used as the injectable material for cartilage regeneration therapies.
中文翻译：
混合蛋白-糖胺聚糖水凝胶促进软骨干细胞分化
明胶-透明质酸（Gel-HA）混合水凝胶因其能够模仿细胞外基质的结构而被提议作为组织工程的基质。我们的目的是探索凝胶和透明质酸的酪胺缀合物，产生可注射的水凝胶，是否能够诱导封装的人间充质干细胞的特定表型，而不需要生长因子。虽然纯凝胶允许良好的细胞粘附而没有显着的分化，并且纯HA触发软骨形成分化而没有细胞扩散，但杂交体，尤其是富含HA的杂交体，促进软骨形成分化以及细胞增殖和粘附。观察到软骨形成标记物如聚集蛋白聚糖、SOX-9、II 型胶原和糖胺聚糖的分泌，而在生长培养基中两周后不存在成骨、肌源和脂肪形成标记物（分别为 RUNX2、肌节肌球蛋白和脂蛋白脂肪酶） 。最有前途的软骨形成基质似乎是含有 70% HA 和 30% 凝胶的混合物，因为它是这里测试的所有组合物中具有最佳机械性能的材料，同时，它提供了适合平衡细胞粘附的环境和软骨分化。因此，它代表了一种很有潜力用作软骨再生治疗的可注射材料的系统。
更新日期：2017-11-07
中文翻译：
混合蛋白-糖胺聚糖水凝胶促进软骨干细胞分化
明胶-透明质酸（Gel-HA）混合水凝胶因其能够模仿细胞外基质的结构而被提议作为组织工程的基质。我们的目的是探索凝胶和透明质酸的酪胺缀合物，产生可注射的水凝胶，是否能够诱导封装的人间充质干细胞的特定表型，而不需要生长因子。虽然纯凝胶允许良好的细胞粘附而没有显着的分化，并且纯HA触发软骨形成分化而没有细胞扩散，但杂交体，尤其是富含HA的杂交体，促进软骨形成分化以及细胞增殖和粘附。观察到软骨形成标记物如聚集蛋白聚糖、SOX-9、II 型胶原和糖胺聚糖的分泌，而在生长培养基中两周后不存在成骨、肌源和脂肪形成标记物（分别为 RUNX2、肌节肌球蛋白和脂蛋白脂肪酶） 。最有前途的软骨形成基质似乎是含有 70% HA 和 30% 凝胶的混合物，因为它是这里测试的所有组合物中具有最佳机械性能的材料，同时，它提供了适合平衡细胞粘附的环境和软骨分化。因此，它代表了一种很有潜力用作软骨再生治疗的可注射材料的系统。
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