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Hepatogenic Supported Differentiation of Mesenchymal Stem Cells in a Lactobionic Acid-Conjugated Thermogel
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acsmacrolett.7b00802 Hyo Jung Moon 1 , Hyun Jung Lee 1 , Madhumita Patel 1 , Sohee Park 1 , Seo Hee Chang 1 , Byeongmoon Jeong 1
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acsmacrolett.7b00802 Hyo Jung Moon 1 , Hyun Jung Lee 1 , Madhumita Patel 1 , Sohee Park 1 , Seo Hee Chang 1 , Byeongmoon Jeong 1
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To investigate the effect of receptor substrate of target cells on stem cell differentiation, lactobionic acid-conjugated poly[(propylene glycol)-b-(ethylene glycol)-b-(propylene glycol)]-poly(l-alanine) (LB-PLX-PA) was synthesized, and then thermogelling systems consisting of LB-PLX-PA and PLX-PA in a ratio of 0/100 (LB-0), 5/95 (LB-5), and 20/80 (LB-20) were constructed as an injectable three-dimensional scaffold toward hepatogenic differentiation of tonsil-derived mesenchymal stem cells (TMSCs). Modulus of LB-0, LB-5, and LB-20 increased to 500–800 Pa at 37 °C (gel) due to the heat induced sol-to-gel transition of the systems during which TMSCs were incorporated into the gel. Based on biomarker expressions and hepatic biofunctions of the differentiated cells, the receptor substrate (LB)–conjugated bioactive thermogel provides compatible microenvironments for the differentiated cells, and thus gives pronounced positive results on the differentiation of the stem cells into target cells during three-dimensional culture, compared with a passive thermogel.
中文翻译：
乳糖酸共轭热凝胶中间充质干细胞的肝源支持分化
为了研究靶细胞受体底物对干细胞分化的影响，乳糖酸共轭聚[(丙二醇) -b- (乙二醇) -b- (丙二醇)]-聚( l-丙氨酸) (LB-PLX-PA) 被合成，然后以 0/100 (LB-0)、5/95 (LB-5) 的比例由 LB-PLX-PA 和 PLX-PA 组成的热凝胶体系，和 20/80 (LB-20) 被构建为可注射的三维支架，用于扁桃体来源的间充质干细胞 (TMSCs) 的肝分化。LB-0、LB-5 和 LB-20 的模量在 37 °C（凝胶）下增加到 500-800 Pa，这是由于在 TMSC 掺入凝胶期间系统的热诱导溶胶到凝胶转变。基于分化细胞的生物标志物表达和肝脏生物功能，结合受体底物（LB）的生物活性热凝胶为分化细胞提供了相容的微环境，从而在三维过程中对干细胞向靶细胞的分化提供了显着的阳性结果。文化，
更新日期：2017-11-07
中文翻译：
乳糖酸共轭热凝胶中间充质干细胞的肝源支持分化
为了研究靶细胞受体底物对干细胞分化的影响，乳糖酸共轭聚[(丙二醇) -b- (乙二醇) -b- (丙二醇)]-聚( l-丙氨酸) (LB-PLX-PA) 被合成，然后以 0/100 (LB-0)、5/95 (LB-5) 的比例由 LB-PLX-PA 和 PLX-PA 组成的热凝胶体系，和 20/80 (LB-20) 被构建为可注射的三维支架，用于扁桃体来源的间充质干细胞 (TMSCs) 的肝分化。LB-0、LB-5 和 LB-20 的模量在 37 °C（凝胶）下增加到 500-800 Pa，这是由于在 TMSC 掺入凝胶期间系统的热诱导溶胶到凝胶转变。基于分化细胞的生物标志物表达和肝脏生物功能，结合受体底物（LB）的生物活性热凝胶为分化细胞提供了相容的微环境，从而在三维过程中对干细胞向靶细胞的分化提供了显着的阳性结果。文化，
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