Crosslinks and conjugation: O⁶-Alkylguanine DNA alkyltransferases (AGTs) can act on O⁶-alkyl 2′-deoxyguanosine and O⁴-alkyl thymidine DNA lesions. We show the ability of the human AGT to efficiently repair intrastrand crosslinks, which can be used to generate covalent complexes. We synthesized IaCL DNA with ethylene or propylene linkages, with the latter being moderately repaired by human AGT.

中文翻译：

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链内交联的DNA的AGT活性是由亚烷基接头调节的。

交联和缀合：O ^6-烷基鸟嘌呤DNA烷基转移酶（AGT）可以作用于O ^6-烷基2'-脱氧鸟苷和O ^4-烷基胸苷DNA损伤。我们展示了人类AGT有效修复链内交联的能力，可用于生成共价复合物。我们合成了具有乙烯或丙烯键的IaCL DNA，后者被人AGT适度修复。