Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins,Faraday Discussions

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Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins
Faraday Discussions ( IF 3.3 ) Pub Date : 2017-11-06 , DOI: 10.1039/c7fd00187h
Shaina Dhamija _{1,

2,

3,

4} , Bhisham Thakur _{2,

3,

4,

5} , Purnananda Guptasarma _{2,

3,

4,

5} , Arijit K. De _{1,

2,

3,

4}

Affiliation

Fluorescent proteins exhibit interesting excited state photochemistry, leading to bright fluorescence emission that renders their versatile biological role and wide use as biomarkers. A molecular-level mechanism of the excited state dynamics is desirable to pinpoint the origin of the bright fluorescence of these proteins. Here we present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual fluorescence emission upon UV excitation. Based on our studies, we propose that the unique nature of the potential energy surface is responsible for the observation of minor fluorescence in Venus which is not seen in wild type GFP.

中文翻译：

探索金星的激发态动力学：荧光蛋白双重发射的起源

荧光蛋白表现出令人感兴趣的激发态光化学，导致发出明亮的荧光，从而使其具有多种生物学作用并广泛用作生物标记物。需要激发态动力学的分子水平机制来查明这些蛋白质的明亮荧光的起源。在这里，我们目前对黄色荧光蛋白变体金星进行研究，并研究在紫外线激发下双重荧光发射背后的光物理。根据我们的研究，我们认为势能表面的独特性质是观察金星中较小荧光的原因，而在野生型GFP中则看不到。

更新日期：2018-04-17

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