Cultivation of microbial consortia provides low-complexity communities that can serve as tractable models to understand community dynamics. Time-resolved metagenomics demonstrated that an aerobic cellulolytic consortium cultivated from compost exhibited community dynamics consistent with the definition of an endogenous heterotrophic succession. The genome of the proposed pioneer population, 'Candidatus Reconcilibacillus cellulovorans', possessed a gene cluster containing multidomain glycoside hydrolases (GHs). Purification of the soluble cellulase activity from a 300litre cultivation of this consortium revealed that ~70% of the activity arose from the 'Ca. Reconcilibacillus cellulovorans' multidomain GHs assembled into cellulase complexes through glycosylation. These remarkably stable complexes have supramolecular structures for enzymatic cellulose hydrolysis that are distinct from cellulosomes. The persistence of these complexes during cultivation indicates that they may be active through multiple cultivations of this consortium and act as public goods that sustain the community. The provision of extracellular GHs as public goods may influence microbial community dynamics in native biomass-deconstructing communities relevant to agriculture, human health and biotechnology.

中文翻译：

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细菌先驱者产生纤维素酶复合物，该复合物通过社区演替而持续存在。

微生物群落的培养提供了低复杂度的群落，可以作为易于理解的模型来了解群落动态。时间分辨的宏基因组学证明，从堆肥中培养的好氧纤维素分解体表现出与内源异养演替的定义一致的群落动态。拟议的先驱群体“ Candidatus reconcilibacillus cellulovorans”的基因组具有一个包含多域糖苷水解酶（GHs）的基因簇。从该财团的300升培养物中纯化可溶性纤维素酶的活性表明，约70％的活性来自'Ca。纤维素酶调节蛋白的多结构域GH通过糖基化组装成纤维素酶复合物。这些非常稳定的复合物具有不同于纤维素体的用于酶促纤维素水解的超分子结构。这些复合物在耕种过程中的持续存在表明它们可能通过该财团的多种耕种而活跃起来，并作为维持社区的公共物品。提供作为公共产品的细胞外生长激素可能会影响与农业，人类健康和生物技术有关的原生生物解构群落中的微生物群落动态。