The expression levels of glycans on the surfaces of cancer and normal cells show different, however, this difference is not noticeable enough to distinguish them directly. So, herein, based on the targeted molecular recognition of the glycans on cell surfaces by 4-mercaptophenyl boronic acid (MPBA), a novel surface-enhanced Raman scattering (SERS) nanoprobe ([email protected]) was prepared by inducing controllable assembly of MPBA decorated Ag nanoparticles ([email protected]) in a certain level via the bridge of glucose to amplify such a limited difference in SERS measurements. On the basis of the aggregation-induced 3D SERS hot spot effect, this multi-particle nanoprobe possesses over 10 times stronger SERS enhancement ability than the individual [email protected] As the different sialic acid (SA) expression on the surfaces of cancer and normal cells led to the different accumulation of [email protected], the results we obtained (mean intensities recorded from five cells) indicate the SA amounts on two kinds of cells can provide 5–7 times signal contrast grade in SERS band intensities (P < 0.001). Compared with the monodispersed nanoprobe, our developed nanoprobe amplifies the SA expression difference on cell surfaces and supports high sensitivity for cancer cell recognition, which might be useful in providing highly effective recognition of the edges of tumor tissues in clinic field.

癌症和正常细胞表面上聚糖的表达水平显示出差异，但是这种差异不足以直接区分它们。因此，在本文中，基于4-巯基苯基硼酸（MPBA）对细胞表面聚糖的靶向分子识别，通过诱导可控制的组装来制备新型的表面增强拉曼散射（SERS）纳米探针（[电子邮件保护]）。 MPBA通过葡萄糖桥来放大SERS测量中的这种有限差异。在聚集诱导的3D SERS热点效应的基础上，这种多粒子纳米探针的SERS增强能力是个体的10倍以上。细胞导致[电子邮件保护]的不同积累，我们获得的结果（从五个细胞记录的平均强度）表明，两种细胞上的SA含量可以在SERS谱带强度中提供5-7倍的信号对比度等级（P <0.001） ）。与单分散纳米探针相比，我们开发的纳米探针可放大SA在细胞表面的表达差异，并支持对癌细胞识别的高度敏感性，