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Separation of aflatoxin B1 from synthetic physiological fluids using talc and diatomite: Kinetic and isotherm aspects
Journal of Chromatography B ( IF 3 ) Pub Date : 2017-11-04 , DOI: 10.1016/j.jchromb.2017.11.001
Myroslav Sprynskyy , Iwona Krzemień-Konieczka , Renata Gadzała-Kopciuch , Bogusław Buszewski

The objective of the study was to examine adsorption of the aflatoxin B1 from synthetic gastric fluid and synthetic intestinal fluid by talc, raw and calcined diatomite. The kinetic and equilibrium adsorption processes were studied in the batch adsorption experiments applying high performance liquid chromatography for the aflatoxin B1 determination. The kinetic study showed a very fast adsorption of the aflatoxin B1 onto the selected adsorbents from the both physiological fluids with reaching equilibrium within 1–15 min. The aflatoxin B1 was almost completely adsorbed in initial linear step of the kinetic process that can be described well by the zero-order kinetics model. The experimental data of the equilibrium adsorption were characterized using the Langmuir and Freundlich isotherm models. The high adsorption effectiveness was found in a range of 90%–100% and 60%–100% for the diatomite samples and the talc respectively at the initial concentrations of the aflatoxin B1 as 31–300 ng/mL. The possible mechanisms of the aflatoxin adsorption onto the used mineral adsorbents are also discussed in the work.



中文翻译:

使用滑石粉和硅藻土从合成生理液中分离黄曲霉毒素B1:动力学和等温线方面

该研究的目的是研究滑石粉,生硅藻土和煅烧硅藻土对合成胃液和合成肠液中黄曲霉毒素B1的吸附。在间歇吸附实验中,采用高效液相色谱法测定黄曲霉毒素B1的动力学和平衡吸附过程进行了研究。动力学研究表明,黄曲霉毒素B1可以非常快速地吸附到两种生理液中选定的吸附剂上,并在1-15分钟内达到平衡。黄曲霉毒素B1在动力学过程的初始线性步骤中几乎完全吸附,可以通过零级动力学模型很好地描述。使用Langmuir和Freundlich等温模型对平衡吸附的实验数据进行了表征。在黄曲霉毒素B1的初始浓度为31-300 ng / mL时,硅藻土样品和滑石粉的高吸附效率分别为90%–100%和60%–100%。在工作中还讨论了黄曲霉毒素吸附到用过的矿物吸附剂上的可能机理。

更新日期:2017-11-04
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