In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50 × 4.6 mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N,N-dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis, scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively. Results showed that the homemade monolith occupied relatively uniform pore structure, low back pressure, and enhanced selectivity for proteins in complex bio-samples. The present work described a simple and efficient method for “fractionation separation” of human plasma proteins, and it is a promising separation method for complex bio-samples in proteomic research.

在这项工作中，通过使用铁卟啉和甲基丙烯酸丁酯作为共聚单体，乙二醇二甲基丙烯酸酯作为交联剂，乙二醇，异丙基，通过自由基聚合在不锈钢柱（50×4.6 mm内径）范围内制造了聚合物整体柱。醇和N，N-二甲基甲酰胺作为三致孔剂，过氧化苯甲酰和N，N-二甲基苯胺作为引发剂。所得的整体柱分别通过元素分析，扫描电子显微镜，氮吸附BET表面积和压汞法进行了表征。结果表明，自制整料占据了相对均匀的孔结构，低背压，并增强了复杂生物样品中蛋白质的选择性。本工作描述了一种简单有效的“分离血浆蛋白”的方法，对于蛋白质组学研究中的复杂生物样品，这是一种很有前途的分离方法。