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Monocore vs. multicore magnetic iron oxide nanoparticles: uptake by glioblastoma cells and efficiency for magnetic hyperthermia†
Molecular Systems Design & Engineering ( IF 3.2 ) Pub Date : 2017-11-03 00:00:00 , DOI: 10.1039/c7me00061h Gauvin Hemery 1, 2, 3, 4, 5 , Coralie Genevois 6, 7, 8, 9 , Franck Couillaud 6, 7, 8, 9 , Sabrina Lacomme 9, 10, 11, 12, 13 , Etienne Gontier 9, 10, 11, 12, 13 , Emmanuel Ibarboure 1, 2, 3, 4, 5 , Sébastien Lecommandoux 1, 2, 3, 4, 5 , Elisabeth Garanger 1, 2, 3, 4, 5 , Olivier Sandre 1, 2, 3, 4, 5
Molecular Systems Design & Engineering ( IF 3.2 ) Pub Date : 2017-11-03 00:00:00 , DOI: 10.1039/c7me00061h Gauvin Hemery 1, 2, 3, 4, 5 , Coralie Genevois 6, 7, 8, 9 , Franck Couillaud 6, 7, 8, 9 , Sabrina Lacomme 9, 10, 11, 12, 13 , Etienne Gontier 9, 10, 11, 12, 13 , Emmanuel Ibarboure 1, 2, 3, 4, 5 , Sébastien Lecommandoux 1, 2, 3, 4, 5 , Elisabeth Garanger 1, 2, 3, 4, 5 , Olivier Sandre 1, 2, 3, 4, 5
Affiliation
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PEGylated magnetic iron oxide nanoparticles (IONPs) were synthesised with the aim to provide proof of concept results of remote cancer cell killing by magnetic fluid hyperthermia. The IONPs were produced by the polyol synthetic route also called the “forced hydrolysis pathway”, yielding highly superparamagnetic, readily-dispersible, and biocompatible IONPs. As shown previously, adjusting the parameters of the reaction led to either monocore or multicore IONPs, with an on-demand morphology and magnetic properties. Polyethylene glycol (PEG) was grafted onto the nanoparticles in a single final step, using a phosphonic acid-terminated PEG synthesised separately, a strategy named “convergent”. The magnetic properties of the IONPs were preserved in physiological media, thanks to this biocompatible shell. The interaction of the PEGylated IONPs with a glioblastoma cell line was studied, from the stability of IONPs in an appropriate cell culture medium to the remotely magnetically triggered cell death. Cellular internalisation of the IONPs was studied, along with their fate after application of an alternating magnetic field (AMF). This investigation highlights the superior efficiency of multicore (nanoflowers) vs. monocore (nanospheres) IONPs for magnetic hyperthermia, leading to 80% cancer cell death in medically translatable conditions.
中文翻译：
单核与多核磁性氧化铁纳米颗粒：胶质母细胞瘤细胞摄取和磁热疗效率†
合成了聚乙二醇化的磁性氧化铁纳米粒子（IONPs），目的是提供通过磁流体高温杀死癌细胞的概念结果的证据。IONP是通过多元醇合成途径（也称为“强制水解途径”）生产的，可产生高度超顺磁性，易分散且具有生物相容性的IONP。如前所示，调整反应参数会导致单核或多核IONP，它们具有按需的形态和磁性。使用单独合成的膦酸封端的PEG，在单个最终步骤中将聚乙二醇（PEG）接枝到纳米颗粒上，该策略称为“收敛”。由于这种生物相容性壳层，IONPs的磁性得以保留在生理介质中。研究了聚乙二醇化IONP与胶质母细胞瘤细胞系的相互作用，从IONP在合适的细胞培养基中的稳定性到远程磁触发的细胞死亡。研究了IONP的细胞内在化及其在施加交变磁场（AMF）后的命运。这项调查凸显了多核（纳米）的卓越效率磁热疗与单核（纳米球）IONP相比，在可医学翻译的条件下导致80％的癌细胞死亡。
更新日期：2017-11-03
中文翻译：
单核与多核磁性氧化铁纳米颗粒：胶质母细胞瘤细胞摄取和磁热疗效率†
合成了聚乙二醇化的磁性氧化铁纳米粒子（IONPs），目的是提供通过磁流体高温杀死癌细胞的概念结果的证据。IONP是通过多元醇合成途径（也称为“强制水解途径”）生产的，可产生高度超顺磁性，易分散且具有生物相容性的IONP。如前所示，调整反应参数会导致单核或多核IONP，它们具有按需的形态和磁性。使用单独合成的膦酸封端的PEG，在单个最终步骤中将聚乙二醇（PEG）接枝到纳米颗粒上，该策略称为“收敛”。由于这种生物相容性壳层，IONPs的磁性得以保留在生理介质中。研究了聚乙二醇化IONP与胶质母细胞瘤细胞系的相互作用，从IONP在合适的细胞培养基中的稳定性到远程磁触发的细胞死亡。研究了IONP的细胞内在化及其在施加交变磁场（AMF）后的命运。这项调查凸显了多核（纳米）的卓越效率磁热疗与单核（纳米球）IONP相比，在可医学翻译的条件下导致80％的癌细胞死亡。
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