3,5 – Dimethylpyrazolium glyceroborate is a nitrification inhibitor (a member of pyrazole derivatives) used for the fixation of nitrogen into the soil. In this study, an HPLC-DAD method was developed and validated for determination of 3,5 – dimethylpyrazole in order to determine 3,5 – dimethylpyrazolium glyceroborate in fertilizer samples. For method development, analytical parameters like type of eluent solution and column filling material and device parameters like eluent flow rate, column oven temperature and measurement wavelength were all optimized. For method validation, implementations were performed for linearity, limit of detection (LOD), limit of quantification (LOQ), specificity, stability, intra- and inter-day precision and accuracy. The developed and validated method was used for inhibitor detection in nitrogenous fertilizers. Sample analyses were performed with 95.6–103.3% recovery rates and 0–4.61% relative errors.

3,5 –甘油硼酸二甲基吡唑鎓是一种硝化抑制剂（吡唑衍生物的一部分），用于将氮固定在土壤中。在这项研究中，开发了一种HPLC-DAD方法并经过验证，可用于测定肥料样品中的3,5-二甲基吡唑甘油硼酸酯。为了进行方法开发，分析参数（如洗脱液和柱填充材料的类型）以及设备参数（如洗脱液流速，柱箱温度和测量波长）均已优化。为了进行方法验证，对线性，检测限（LOD），定量限（LOQ），特异性，稳定性，日内和日间精度和准确性进行了实施。经过开发和验证的方法用于氮肥中抑制剂的检测。