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Inactivation of a wild isolated Klebsiella pneumoniae by photo-chemical processes: UV-C, UV-C/H2O2 and UV-C/H2O2/Fe3+
Catalysis Today ( IF 5.2 ) Pub Date : 2017-10-31 , DOI: 10.1016/j.cattod.2017.10.043
Silvio Aguilar , Daniel Rosado , Javier Moreno-Andrés , Luis Cartuche , Darío Cruz , Asunción Acevedo-Merino , Enrique Nebot

Shrimp (Litopenaeus vannamei) farming is an important economic activity in several countries. Pathogens in shrimp farms and its effluents pose a potential hazard for both humans and shrimps. Wild strains of bacteria were characterized in a shrimp farm, and Klebsiella pneumoniae was chosen as a good indicator due to its presence in the pond and the effluent and its resistance to antibiotics. Different photochemical processes (UV/H2O2, UV/H2O2/Fe3+) were tested for inactivation of wild isolated K. pneumoniae and compared to UV-C radiation. By kinetic modelling, a kmax equal to 0.43 s−1 was obtained for UV-C treatment. After optimizing the cited processes, ranging [H2O2]: 10–30 mg·l−1; an optimal [H2O2] of 10 mg·l−1 was found, increasing kmax on 13.63% compared to UV-C. This optimal concentration was tested for UV/H2O2/Fe3+ process; ranging [Fe3+]: 2–20 mg·l−1. The highest yield was obtained by a [H2O2]:[Fe3+] = 10:2, which leads to 4-Log reduction in 12.88 s of treatment. Moreover, resistance of K. pneumoniae was compared to Escherichia coli. The latter proved to be more sensitive despite its similar cellular structure. Results suggested that the photochemical processes could enhance disinfection efficiency, especially for photo-assisted Fenton-like process in most resistant bacteria.



中文翻译:

通过光化学过程灭活野生的分离的肺炎克雷伯菌:UV-C,UV-C / H 2 O 2和UV-C / H 2 O 2 / Fe 3+

虾养殖(南美对虾)在一些国家是一项重要的经济活动。虾场及其废水中的病原体对人类和虾均构成潜在危害。在虾场中对野生菌株进行了鉴定,由于其在池塘中和废水中的存在及其对抗生素的抗性,因此选择了肺炎克雷伯菌为良好的指示剂。测试了不同的光化学过程(UV / H 2 O 2,UV / H 2 O 2 / Fe 3+)对野生分离的肺炎克雷伯菌的灭活作用,并将其与UV-C辐射进行了比较。通过动力学建模,ak max等于0.43 s -1获得用于UV-C处理的产物。优化所引用的方法后,范围为[H 2 O 2 ]:10–30 mg·l -1 ; 发现最佳[H 2 O 2 ]为10 mg·l -1,与UV-C相比,k max增加13.63%。测试了UV / H 2 O 2 / Fe 3+工艺的最佳浓度;范围[Fe 3+ ]:2–20 mg·l -1。通过[H 2 O 2 ]:[Fe 3+ ] = 10:2获得了最高的产率,这导致在12.88 s的处理过程中4-Log降低。此外,肺炎克雷伯菌的耐药大肠杆菌进行了比较尽管后者具有相似的细胞结构,但事实证明后者更为敏感。结果表明,光化学过程可以提高消毒效率,特别是对于大多数抗性细菌的光辅助Fenton样过程。

更新日期:2017-10-31
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