Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02 μg L⁻¹ and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0 μg L⁻¹ anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.

特异性单克隆抗体与基于琼脂糖凝胶的磁珠偶联并首次使用。该方法适用于从水中预浓缩抗毒素a，随后通过离子迁移谱（IMS）测定。分散磁免疫亲和（d-MagIA）提取方法提供了0.02μgL ^-1的检测限和令人满意的精度，相对标准偏差低于15％。以0.5、1.0和5.0μgL ^-1 anatoxin-a评估回收率，定量值为91％至115％。此外，通过使用已开发的d-MagIA提取物与IMS测定相结合，完全避免了苯丙氨酸的同量异位干扰。