Exploratory metabolomics study of the experimental opisthorchiasis in a laboratory animal model (golden hamster, Mesocricetus auratus),PLOS Neglected Tropical Diseases

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Exploratory metabolomics study of the experimental opisthorchiasis in a laboratory animal model (golden hamster, Mesocricetus auratus)
PLOS Neglected Tropical Diseases ( IF 3.4 ) Pub Date : 2017-10-31 , DOI: 10.1371/journal.pntd.0006044
Daria A Kokova ₁ , Sarantos Kostidis ₂ , Judit Morello _{2,

3} , Nataly Dementeva ₄ , Ekaterina A Perina ₅ , Vladimir V Ivanov ₅ , Ludmila M Ogorodova ₆ , Aleksey E Sazonov ₄ , Irina V Saltykova ₃ , Oleg A Mayboroda _{2,

4}

Affiliation

Background

Opisthorchiasis is a parasitic infection caused by the liver flukes of the Opisthorchiidae family. Both experimental and epidemiological data strongly support a role of these parasites in the etiology of the hepatobiliary pathologies and an increased risk of intrahepatic cholangiocarcinoma. Understanding a functional link between the infection and hepatobiliary pathologies requires a detailed description a host-parasite interaction on different levels of biological regulation including the metabolic response on the infection. The last one, however, remains practically undocumented. Here we are describing a host response on Opisthorchiidae infection using a metabolomics approach and present the first exploratory metabolomics study of an experimental model of O. felineus infection.

Methodology and Principal findings

We conducted a Nuclear Magnetic Resonance (NMR) based longitudinal metabolomics study involving a cohort of 30 animals with two degrees of infection and a control group. An exploratory analysis shows that the most noticeable trend (30% of total variance) in the data was related to the gender differences. Therefore further analysis was done of each gender group separately applying a multivariate extension of the ANOVA—ASCA (ANOVA simultaneous component analysis). We show that in the males the infection specific time trends are present in the main component (43.5% variance), while in the females it is presented only in the second component and covers 24% of the variance. We have selected and annotated 24 metabolites associated with the observed effects and provided a physiological interpretation of the findings.

Conclusions

The first exploratory metabolomics study an experimental model of O. felineus infection is presented. Our data show that at early stage of infection a response of an organism unfolds in a gender specific manner. Also main physiological mechanisms affected appear rather nonspecific (a status of the metabolic stress) the data provides a set of the hypothesis for a search of the more specific metabolic markers of the Opisthorchiidae infection.

中文翻译：

实验动物模型（金仓鼠、Mesocricetus auratus）中实验性阿虫病的探索性代谢组学研究

背景

后睾吸虫病是由后睾吸虫科肝吸虫引起的寄生虫感染。实验和流行病学数据都强烈支持这些寄生虫在肝胆病理学病因学中的作用以及肝内胆管癌风险增加。了解感染与肝胆病理之间的功能联系需要详细描述不同水平的生物调节（包括感染的代谢反应）上的宿主-寄生虫相互作用。然而，最后一个实际上仍然没有记录。在这里，我们使用代谢组学方法描述了宿主对 Opisthorchiidae 感染的反应，并提出了O实验模型的第一个探索性代谢组学研究。猫科动物感染。

方法论和主要发现

我们进行了一项基于核磁共振 (NMR) 的纵向代谢组学研究，涉及 30 只具有两级感染的动物和对照组。探索性分析表明，数据中最显着的趋势（总方差的 30%）与性别差异有关。因此，应用方差分析-ASCA（方差分析同时成分分析）的多变量扩展，分别对每个性别组进行了进一步分析。我们发现，在男性中，感染特定时间趋势出现在主要成分中（43.5% 方差），而在女性中，感染特定时间趋势仅出现在第二个成分中，覆盖了 24% 的方差。我们选择并注释了 24 种与观察到的效应相关的代谢物，并对研究结果提供了生理学解释。