With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on average, binding of ∼11.5 histone H1 molecules causes one linking number change in circular polynucleosomes in the presence of 1.5 mM spermidine. When concentrations of spermidine decreases or increases, these linking number differences decrease significantly. It is therefore evident that linking number changes caused by histone H1 are spermidine concentration-dependent.

为了发现组蛋白H1对DNA的连接数目变化的贡献，在我们的研究中进行了确定无组蛋白H1的环状多核小体与组蛋白H1结合的环状多核小体之间的连接数差异的测定。我们的结果表明，平均而言，在1.5 mM亚精胺存在下，约11.5个组蛋白H1分子的结合会导致环状多核小体发生一个连接数变化。当亚精胺的浓度降低或增加时，这些连接数目的差异显着降低。因此，很明显，由组蛋白H1引起的连接数变化是亚精胺浓度依赖性的。