A new trichodermamide-like alkaloid, N-Me-trichodermamide B (compound 1), was isolated from a marine fungus Penicillium janthinellum HDN13-309. The structure and absolute configuration of compound 1 were determined by extensive NMR analysis and the modified Mosher’s method. This new alkaloid exhibited cellular protection from the H₂O₂-induced oxidative damage, and the mechanism study revealed that this antioxidant activity was regulated through Nrf2-mediated signaling pathway in HaCaT human keratinocytes. In addition, the inhibitor of p38 abrogated compound 1-induced phosphorylation of p38, up-expression of HO-1, and the nuclear localization of Nrf2. As a result, it suggested that this new alkaloid-induced antioxidant signaling pathway might be initiated through activation of p38.

一种新的trichodermamide样生物碱，ñ -Me-trichodermamide B（化合物1），是从海洋真菌中分离微紫青霉HDN13-309。化合物1的结构和绝对构型通过广泛的NMR分析和改进的Mosher方法确定。这种新的生物碱对H ₂ O _2具有细胞保护作用诱导的氧化损伤，其机理研究表明，该抗氧化活性是通过HaCaT人角质形成细胞中Nrf2介导的信号传导途径调节的。此外，p38抑制剂消除了化合物1诱导的p38磷酸化，HO-1的过表达以及Nrf2的核定位。结果表明，这种新的生物碱诱导的抗氧化剂信号传导途径可能是通过激活p38引发的。