Fluorapatite (FA) nanoparticles were synthesized via an one-step and green pathway by employing the deep eutectic solvent based on choline chloride-CaCl₂.6H₂O. In an innovative approach, the eutectic solvent was chosen in such a way to act as solvent, reactant and template, simultaneously. The results obtained from characterization of the as-synthesized nanoparticles revealed the formation of FA nanoparticles having average crystal size of 26 nm, percent crystallinity of 99%, mean particle size of 30 nm, and high crystal, elemental, and structural purity. In vitro study was conducted by immersion of FA nanoparticles in SBF solution. The results obtained by SEM, EDS, FTIR, and Raman analysis confirmed the formation of bioactive apatite layer on surface of the nanoparticles after 7 days of immersion. The MTT assay and acridine orange staining test using hMSCs suggested the non-toxicity and osteogenic ability of as-synthesized FA nanoparticles. Based on the proposed synthesis mechanism, the deep eutectic system played a ternary role in synthesis of FA nanoparticles providing the synthesis environment, reactive calcium sites, and steric-electrostatic stabilization by which it can control the growth of as-formed FA particles in the nanometer size-range.

采用基于氯化胆碱-CaCl ₂ .6H ₂ O的深层低共熔溶剂，通过一步法和绿色途径合成了氟磷灰石（FA）纳米颗粒。在一种创新方法中，选择低共熔溶剂的作用是溶剂，反应物和模板。从合成后的纳米颗粒的表征获得的结果表明，FA纳米颗粒的形成具有26 nm的平均晶体尺寸，99％的结晶度百分比，30 nm的平均粒度以及高晶体，元素和结构纯度。体外通过将FA纳米颗粒浸入SBF溶液中进行了研究。通过SEM，EDS，FTIR和拉曼分析获得的结果证实了浸没7天后纳米颗粒表面上形成了生物活性磷灰石层。使用hMSC的MTT分析和and啶橙染色测试表明，合成的FA纳米颗粒具有无毒和成骨能力。基于提出的合成机理，深共晶体系在FA纳米颗粒的合成中发挥了三元作用，提供了合成环境，反应性钙位和空间静电稳定性，从而可以控制纳米形式的FA颗粒的生长。尺寸范围。