Permeable Protein-Loaded Polymersome Cascade Nanoreactors by Polymerization-Induced Self-Assembly,ACS Macro Letters

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Permeable Protein-Loaded Polymersome Cascade Nanoreactors by Polymerization-Induced Self-Assembly
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-10-31 00:00:00 , DOI: 10.1021/acsmacrolett.7b00725
Lewis D Blackman ₁ , Spyridon Varlas ₁ , Maria C Arno ₁ , Alice Fayter ₁ , Matthew I Gibson _{1,

2} , Rachel K O'Reilly ₁

Affiliation

Enzyme loading of polymersomes requires permeability to enable them to interact with the external environment, typically requiring addition of complex functionality to enable porosity. Herein, we describe a synthetic route toward intrinsically permeable polymersomes loaded with functional proteins using initiator-free visible light-mediated polymerization-induced self-assembly (photo-PISA) under mild, aqueous conditions using a commercial monomer. Compartmentalization and retention of protein functionality was demonstrated using green fluorescent protein as a macromolecular chromophore. Catalytic enzyme-loaded vesicles using horseradish peroxidase and glucose oxidase were also prepared and the permeability of the membrane toward their small molecule substrates was revealed for the first time. Finally, the interaction of the compartmentalized enzymes between separate vesicles was validated by means of an enzymatic cascade reaction. These findings have a broad scope as the methodology could be applied for the encapsulation of a large range of macromolecules for advancements in the fields of nanotechnology, biomimicry, and nanomedicine.

中文翻译：

聚合诱导自组装可渗透蛋白质负载聚合物体级联纳米反应器

聚合物囊泡的酶负载需要渗透性，以使它们能够与外部环境相互作用，通常需要添加复杂的功能以实现多孔性。在此，我们描述了一种合成路线，使用商用单体在温和的水性条件下使用无引发剂的可见光介导的聚合诱导自组装（photo-PISA）来合成装载有功能性蛋白质的本质渗透性聚合物囊泡。使用绿色荧光蛋白作为大分子发色团证明了蛋白质功能的区室化和保留。还使用辣根过氧化物酶和葡萄糖氧化酶制备了负载催化酶的囊泡，并首次揭示了膜对其小分子底物的渗透性。最后，通过酶级联反应验证了单独囊泡之间分隔酶的相互作用。这些发现具有广泛的范围，因为该方法可应用于封装多种大分子，以促进纳米技术、仿生学和纳米医学领域的进步。

更新日期：2017-10-31

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