Panax ginseng has been the subject of extensive research on potential medicinal materials. The goal of this study was search the chemical constituents and biological activities of processed Panax ginseng, Korean red ginseng. Our efforts led to the isolation eleven compounds (1–11) including two new compounds 1 and 2 from Korean red ginseng using various chromatographic techniques. Chemical structures of isolated compounds were demonstrated by spectroscopic methods (1D-, 2D-NMR, and HR-ESI-MS). The anti-inflammatory effects of the compounds were investigated by inhibiting IL-6 and TNF-α secretion in LPS-activated RAW264.7 cells. Additionally, the effects of the compounds on the expression of COX-2 and iNOS were examined by Western blotting. Compound 1 significantly reduced the level of proinflammatory cytokines IL-6 and TNF-α secretion in LPS-activated RAW264.7 cells and the expression of COX-2 and iNOS inflammatory enzymes in the cells. These results suggested that compound 1, a new ginsenoside might useful in treatment of inflammation.

人参一直是潜在药用材料广泛研究的对象。本研究的目的是寻找加工后的人参、高丽红参的化学成分和生物活性。我们的努力导致使用各种色谱技术从韩国红参中分离出 11 种化合物 ( 1 – 11 )，包括两种新化合物1和2 。通过光谱方法（1D-、2D-NMR 和 HR-ESI-MS）证明了分离化合物的化学结构。通过抑制 LPS 激活的 RAW264.7 细胞中 IL-6 和 TNF-α 的分泌来研究化合物的抗炎作用。此外，通过蛋白质印迹检查了化合物对 COX-2 和 iNOS 表达的影响。化合物1显着降低LPS激活的RAW264.7细胞中促炎细胞因子IL-6和TNF-α的分泌水平以及细胞中COX-2和iNOS炎症酶的表达。这些结果表明化合物1 （一种新的人参皂苷）可能有助于治疗炎症。