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Full-angle tomographic phase microscopy of flowing quasi-spherical cells†
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-31 00:00:00 , DOI: 10.1039/c7lc00943g Massimiliano M. Villone 1, 2, 3, 4, 5 , Pasquale Memmolo 5, 6, 6, 7, 8 , Francesco Merola 5, 6, 7, 8 , Martina Mugnano 5, 6, 7, 8 , Lisa Miccio 5, 6, 7, 8 , Pier Luca Maffettone 1, 2, 3, 4, 5 , Pietro Ferraro 5, 6, 7, 8
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-31 00:00:00 , DOI: 10.1039/c7lc00943g Massimiliano M. Villone 1, 2, 3, 4, 5 , Pasquale Memmolo 5, 6, 6, 7, 8 , Francesco Merola 5, 6, 7, 8 , Martina Mugnano 5, 6, 7, 8 , Lisa Miccio 5, 6, 7, 8 , Pier Luca Maffettone 1, 2, 3, 4, 5 , Pietro Ferraro 5, 6, 7, 8
Affiliation
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We report a reliable full-angle tomographic phase microscopy (FA-TPM) method for flowing quasi-spherical cells along microfluidic channels. This method lies in a completely passive optical system, i.e. mechanical scanning or multi-direction probing of the sample is avoided. It exploits the engineered rolling of cells while they are flowing along a microfluidic channel. Here we demonstrate significant progress with respect to the state of the art of in-flow TPM by showing a general extension to cells having almost spherical shapes while they are flowing in suspension. In fact, the adopted strategy allows the accurate retrieval of rotation angles through a theoretical model of the cells' rotation in a dynamic microfluidic flow by matching it with phase-contrast images resulting from holographic reconstructions. So far, the proposed method is the first and the only one that permits to get in-flow TPM by probing the cells with full-angle, achieving accurate 3D refractive index mapping and the simplest optical setup, simultaneously. Proof of concept experiments were performed successfully on human breast adenocarcinoma MCF-7 cells, opening the way for the full characterization of circulating tumor cells (CTCs) in the new paradigm of liquid biopsy.
中文翻译：
流动的准球形细胞的全角层析相显微镜†
我们报告了一种可靠的全角断层扫描相显微镜（FA-TPM）方法沿微流体通道流动的准球形细胞。该方法位于完全无源的光学系统中，即避免对样品进行机械扫描或多方向探测。它利用细胞在微流通道中流动时的工程化滚动。在这里，我们通过显示在悬浮状态下流动的几乎球形的细胞的一般扩展，证明了在流入TPM技术方面的重大进展。实际上，所采用的策略通过将其与全息重构产生的相衬图像进行匹配，可以通过动态微流体流中细胞旋转的理论模型来准确检索旋转角度。到目前为止，所提出的方法是第一个也是唯一一个通过以全角度探测单元，同时实现精确的3D折射率映射和最简单的光学设置来获得流入TPM的方法。
更新日期：2017-10-31
中文翻译：
流动的准球形细胞的全角层析相显微镜†
我们报告了一种可靠的全角断层扫描相显微镜（FA-TPM）方法沿微流体通道流动的准球形细胞。该方法位于完全无源的光学系统中，即避免对样品进行机械扫描或多方向探测。它利用细胞在微流通道中流动时的工程化滚动。在这里，我们通过显示在悬浮状态下流动的几乎球形的细胞的一般扩展，证明了在流入TPM技术方面的重大进展。实际上，所采用的策略通过将其与全息重构产生的相衬图像进行匹配，可以通过动态微流体流中细胞旋转的理论模型来准确检索旋转角度。到目前为止，所提出的方法是第一个也是唯一一个通过以全角度探测单元，同时实现精确的3D折射率映射和最简单的光学设置来获得流入TPM的方法。
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