当前位置：
X-MOL 学术
›
ACS Biomater. Sci. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Alternative SiO2 Surface Direct MDCK Epithelial Behavior
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-10-30 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00645 Alan D. Covell 1 , Zheng Zeng 1 , Taylor Mabe 1 , Jianjun Wei 1 , Amy Adamson 2 , Dennis R. LaJeunesse 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-10-30 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00645 Alan D. Covell 1 , Zheng Zeng 1 , Taylor Mabe 1 , Jianjun Wei 1 , Amy Adamson 2 , Dennis R. LaJeunesse 1
Affiliation
|
The mechanical interactions of cells are mediated through adhesive interactions. In this study, we examined the growth, cellular behavior, and adhesion of MDCK epithelial cells on three different SiO2 substrates: amorphous glass coverslips and the silicon oxide layers that grow on ⟨111⟩ and ⟨100⟩ wafers. While compositionally all three substrates are almost similar, differences in surface energy result in dramatic differences in epithelial cell morphology, cell–cell adhesion, cell–substrate adhesion, actin organization, and extracellular matrix (ECM) protein expression. We also observe striking differences in ECM protein binding to the various substrates due to the hydrogen bond interactions. Our results demonstrate that MDCK cells have a robust response to differences in substrates that is not obviated by nanotopography or surface composition and that a cell’s response may manifest through subtle differences in surface energies of the materials. This work strongly suggests that other properties of a material other than composition and topology should be considered when interpreting and controlling interactions of cells with a substrate, whether it is synthetic or natural.
中文翻译：
替代SiO 2表面直接MDCK上皮行为
细胞的机械相互作用是通过粘附相互作用介导的。在这项研究中，我们检查了三种不同SiO 2上MDCK上皮细胞的生长，细胞行为和粘附基板：非晶玻璃盖玻片和在⟨111⟩和⟨100⟩晶片上生长的氧化硅层。尽管三种底物的成分几乎相似，但表面能的差异导致上皮细胞形态，细胞间粘附，细胞底物粘附，肌动蛋白组织和细胞外基质（ECM）蛋白表达发生显着差异。我们还观察到由于氢键相互作用，ECM蛋白与各种底物结合的显着差异。我们的研究结果表明，MDCK细胞对基质的差异具有很强的响应能力，而纳米形貌或表面成分并未消除该缺陷，并且细胞的响应可能通过材料表面能的细微差别体现出来。
更新日期：2017-10-31
中文翻译：
替代SiO 2表面直接MDCK上皮行为
细胞的机械相互作用是通过粘附相互作用介导的。在这项研究中，我们检查了三种不同SiO 2上MDCK上皮细胞的生长，细胞行为和粘附基板：非晶玻璃盖玻片和在⟨111⟩和⟨100⟩晶片上生长的氧化硅层。尽管三种底物的成分几乎相似，但表面能的差异导致上皮细胞形态，细胞间粘附，细胞底物粘附，肌动蛋白组织和细胞外基质（ECM）蛋白表达发生显着差异。我们还观察到由于氢键相互作用，ECM蛋白与各种底物结合的显着差异。我们的研究结果表明，MDCK细胞对基质的差异具有很强的响应能力，而纳米形貌或表面成分并未消除该缺陷，并且细胞的响应可能通过材料表面能的细微差别体现出来。
京公网安备 11010802027423号