Incorporation of a Ligand Peptide for Immune Inhibitory Receptor LAIR‐1 on Biomaterial Surfaces Inhibits Macrophage Inflammatory Responses,Advanced Healthcare Materials

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Incorporation of a Ligand Peptide for Immune Inhibitory Receptor LAIR‐1 on Biomaterial Surfaces Inhibits Macrophage Inflammatory Responses
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-10-30 , DOI: 10.1002/adhm.201700707
Yoon Kyung Kim _{1,

2,

3} , Shu-Hui Chu ₄ , Jessica Y Hsieh _{2,

3} , Cody M Kamoku ₁ , Andrea J Tenner ₄ , Wendy F Liu _{1,

2,

3} , Szu-Wen Wang _{1,

2}

Affiliation

Leukocyte‐associated immunoglobulin‐like receptor‐1 (LAIR‐1) is an inhibitory receptor broadly expressed on immune cells, with its ligands residing within the extracellular matrix protein collagen. In this study, surfaces are modified with a LAIR‐1 ligand peptide (LP), and it is observed that macrophages cultured on LAIR‐1 LP‐conjugated surfaces exhibit significantly reduced secretion of inflammatory cytokines in response to proinflammatory stimuli that reflect an injured environment. These downregulated mediators include TNF‐α, MIP‐1α, MIP‐1β, MIP‐2, RANTES, and MIG. Knockdown of LAIR‐1 using siRNA abrogates this inhibition of cytokine secretion, supporting the specificity of the inhibitory effect to this receptor. These results are the first to demonstrate that integration of LAIR‐1 ligands with biomaterials could suppress inflammatory responses.

中文翻译：

在生物材料表面掺入免疫抑制受体 LAIR-1 的配体肽可抑制巨噬细胞炎症反应

白细胞相关免疫球蛋白样受体-1 (LAIR-1) 是一种在免疫细胞上广泛表达的抑制性受体，其配体位于细胞外基质蛋白胶原蛋白中。在这项研究中，用 LAIR-1 配体肽 (LP) 修饰表面，观察到在 LAIR-1 LP 缀合的表面上培养的巨噬细胞在响应反映受损环境的促炎刺激时表现出炎性细胞因子的分泌显着减少. 这些下调的介质包括 TNF-α、MIP-1α、MIP-1β、MIP-2、RANTES 和 MIG。使用 siRNA 敲低 LAIR-1 消除了对细胞因子分泌的这种抑制，支持了对该受体的抑制作用的特异性。这些结果首次证明 LAIR-1 配体与生物材料的整合可以抑制炎症反应。

更新日期：2017-10-30

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