An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug–Protein Binding,ACS Medicinal Chemistry Letters

当前位置： X-MOL 学术 › ACS Med. Chem. Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug–Protein Binding
ACS Medicinal Chemistry Letters ( IF 3.5 ) Pub Date : 2017-09-28 00:00:00 , DOI: 10.1021/acsmedchemlett.7b00316
Zackary M. Herbst ₁ , Sayaka Shibata ₂ , Erkang Fan ₂ , Michael H. Gelb _{2,

3}

Affiliation

An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fraction of the cost of a commercial, multichamber microdialysis device. Drug–protein binding results are provided, which validates the device.

中文翻译：

一种便宜的内部微透析装置，用于测量药物-蛋白质的结合

描述了一种廉价的内部制造的微透析装置，其适合于测量包括药物候选物的小分子与血清蛋白或其他大分子的结合。该设备基于标准的平衡透析方法来测量与蛋白质结合的低分子量化合物的比例。它由标准的聚丙烯96孔板，透析管和低粘度环氧树脂构成。可以容易地制备该装置，而只需花费商业多腔微透析装置的一小部分费用。提供了药物-蛋白质结合结果，可验证设备。

更新日期：2017-09-28

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11