Quantitative effect-directed profiles of 77 industrially and freshly extracted botanicals like herbs, spices, vegetables and fruits, widely used as food ingredients, dietary supplements or traditional medicine, gave relevant information on their quality. It allows the assessment of food, dietary supplements and phytomedicines with regard to potential health-promoting activities. In contrary to sum parameter assays and targeted analysis, chromatography combined with effect-directed analysis allows fast assignment of single active compounds and evaluation of their contribution to the overall activity, originating from a food or botanical sample. High-performance thin-layer chromatography was hyphenated with UV/Vis/FLD detection and effect-directed analysis, using the 2,2-diphenyl-1-picrylhydrazyl radical, Gram-negative Aliivibrio fischeri, Gram-positive Bacillus subtilis, acetylcholinesterase and tyrosinase assays. Bioactive compounds of interest were eluted using an elution head-based interface and further characterized by electrospray ionization (high-resolution) mass spectrometry. This highly streamlined workflow resulted in a hyphenated HPTLC-UV/Vis/FLD-EDA-ESI⁺/ESI⁻-(HR)MS method. The excellent quantification power of the method was shown on three compounds. For rosmarinic acid, contents ranged from 4.5 mg/g (rooibos) to 32.6 mg/g (rosemary), for kaempferol-3-glucoside from 0.6 mg/g (caraway) to 4.4 mg/g (wine leaves), and for quercetin-3-glucoside from 1.1 mg/g (hawthorn leaves) to 17.7 mg/g (thyme). Three mean repeatabilities (%RSD) over 18 quantifications for the three compounds were ≤2.2% and the mean intermediate precision over three different days (%RSD, n = 3) was 5.2%.

定量分析了77种工业和新鲜提取的植物药（例如草药，香料，蔬菜和水果）的功效，这些植物被广泛用作食品成分，膳食补充剂或传统药物，从而提供了有关其质量的信息。它可以就潜在的促进健康的活动对食物，膳食补充剂和植物药进行评估。与总参数分析和靶向分析相反，色谱结合效果导向分析可以快速分配单一活性化合物并评估其对源自食物或植物样品的总体活性的贡献。通过使用革兰氏阴性的2,2-二苯基-1-吡啶并肼基进行UV / Vis / FLD检测和效果导向分析，对高性能薄层色谱进行连接费氏Aliivibrio fischeri，革兰氏阳性枯草芽孢杆菌，乙酰胆碱酯酶和酪氨酸酶测定法。使用基于洗脱头的界面洗脱感兴趣的生物活性化合物，并进一步通过电喷雾电离（高分辨率）质谱仪进行表征。这种高度简化的工作流程造成了连字符连接的HPTLC-UV /可见光/ FLD-EDA-ESI ⁺ / ESI ^- - （HR）MS方法。该方法在三种化合物上均具有出色的定量能力。迷迭香酸的含量范围为4.5 mg / g（rooibos）至32.6 mg / g（迷迭香），山ka酚3糖苷的含量范围为0.6 mg / g（香菜）至4.4 mg / g（酒叶），槲皮素-3-葡萄糖苷从1.1 mg / g（山楂叶）到17.7 mg / g（百里香）。三种平均重复性（％RSD）三种化合物的18种以上定量结果均≤2.2％，并且在三天内的平均中间精度（％RSD，n  = 3）为5.2％。