A two-dimensional ion chromatography system was developed for the determination of γ-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10 mg L⁻¹, ion exchange chromatography was used in the second dimensional separation via column-switching technique for detection of GHB above 0.08 mg L⁻¹. Under the optimized chromatographic conditions, the ion exclusion and ion exchange chromatography separation system exhibited satisfactory repeatability (RSD < 3.1%, n = 6) and good linearity in the range of 50–1000 mg L⁻¹ and 0.5–100 mg L⁻¹, respectively. By this method, concentrations of GHB in the selected human urine samples were detected in the range of 0–1.57 mg L⁻¹. The urine sample containing 0.89 mg L⁻¹ GHB was selected to evaluate the accuracy; the spiked recoveries of GHB were 95.9-102.8%. The results showed that the two-dimensional ion chromatography system was convenient and practical for the determination of GHB in human urine samples.

开发了一种二维离子色谱系统，用于测定人尿液样品中的γ-羟基丁酸酯（GHB）。离子排斥色谱法在所述第一维分离用于尿矩阵的消除和检测GHB的高于10毫克的L ^-1，在第二维分离通过柱切换技术中使用离子交换色谱法进行检测GHB的上述0.08毫克的L ^{- 1}。在最佳色谱条件下，离子排斥和离子交换色谱分离系统在50–1000 mg L ^-1和0.5–100 mg L ^-1的范围内表现出令人满意的重复性（RSD <3.1％，n = 6）和良好的线性。， 分别。通过这种方法，在选定的人类尿液样本中检测到的GHB浓度在0-1.57 mg L ^-1的范围内。选择含0.89 mg L ^-1 GHB的尿液样本以评估准确性；GHB的加标回收率为95.9-102.8％。结果表明，二维离子色谱系统简便，实用，可用于人尿中GHB的测定。