This study was to evaluate the protective effects of a dietary fiber, konjac glucomannan (KGM) from the plant tuber of Amorphohallus konjac on Bifidobacteria against antibiotic damage. KGM (∼8.8 × 10⁸ Da) was partially degraded with high-intensity ultrasound to KGM-US (∼1.8 × 10⁶ Da) and then hydrolyzed with trifluoroacetic acid (TFA) to KGM-AH (1369 Da). KGM-US (at 5 g/l) showed the most significant protective effect on most bifidobacterial strains against penicillin and streptomycin inhibition, increasing the minimal inhibitory and bactericidal concentration (MIC and MBC) dramatically, and KGM also showed significant effects on enhancing the MBC of enrofloxacin, penicillin, tetracycline and streptomycin. In addition, the adsorbance ability and biofilm improving effects of KGM and degraded KGM products may partially contributed to the protective effects. The results suggested that KGM and ultrasound treated KGM have protective effects for the human gut probiotic bacteria against the damage caused by specific antibiotics.

中文翻译：

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天然和部分降解的魔芋葡甘露聚糖对双歧杆菌对抗生素损害的保护作用

本研究是为了评估从所述的植物块茎一个膳食纤维，魔芋葡甘聚糖（KGM）的保护作用Amorphohallus魔芋上双歧杆菌对抗生素的损害。 高强度超声将KGM（〜8.8×10 ⁸ Da）部分降解为KGM-US（〜1.8×10 ⁶ Da），然后用三氟乙酸（TFA）水解为KGM-AH（1369 Da）。KGM-US（5 g / l）对大多数双歧杆菌菌株显示出最显着的青霉素和链霉素抑制作用，显着提高了最小抑菌和杀菌浓度（MIC和MBC），KGM也显示出对增强MBC的显著作用。恩诺沙星，青霉素，四环素和链霉素。另外，KGM和降解的KGM产物的吸附能力和生物膜改善作用可能部分地起到保护作用。结果表明，KGM和超声处理的KGM对人肠道益生菌具有保护作用，可抵抗特定抗生素引起的损害。