Fluorescent molecular imaging has advanced drastically over the past decade. With the development of high-resolution microscopy techniques and the ability to visualize intracellular molecular events, there is a growing need for new fluorophores to accompany these fast-developing techniques. Therefore, there has been substantial development of alternative fluorophores for single-molecule detection and molecular imaging. These rationally designed fluorophores have infinite possibilities and novel fluorophores are constantly being produced for different applications. This review focuses on the recent developments in novel fluorophores designed for molecular imaging and single-molecule detection. Here, single-molecule imaging, smart fluorescent probes, two-photon microscopy, Förster resonance energy transfer (FRET) and super-resolution microscopy are discussed in detail.

在过去的十年中，荧光分子成像取得了巨大的进步。随着高分辨率显微镜技术的发展和可视化细胞内分子事件的能力，对伴随这些快速发展的技术的新型荧光团的需求日益增长。因此，已经出现了用于单分子检测和分子成像的替代荧光团的实质性发展。这些合理设计的荧光团具有无限的可能性，并且不断为不同的应用生产新颖的荧光团。这篇综述集中在设计用于分子成像和单分子检测的新型荧光团的最新进展。在这里，单分子成像，智能荧光探针，双光子显微镜，