Metal phthalocyanines are promising components in photodynamic therapy. Aluminum phthalocyanine chloride (AlClPc) has been used to treat oral cancer in mice, human carious tissue, lung cancer cells and other conditions. To overcome the high hydrophobicity of AlClPc, phthalocyanine is often encapsulated in nanoformulations. Despite increased usage, little is known about the pharmacokinetics and biodistribution of AlClPc. The aim of this study was the development and validation of a UHPLC-MS method for the determination of AlClPc in solution after extraction from nanoformulations and biological matrices such as plasma and tissue. The described method has been assayed as to selectivity, linearity, limits of detection and quantification, precision and recovery. The present study is the first to describe the behavior of AlClPc in biological matrices with mass spectrometry as well as the first to describe the chromatographic behavior of AlClPc contaminants. Molecular mass analysis identified dechlorination of AlClPc by both LC/MS and MALDI-MS and an adduct formation in LC/MS. The parameters observed indicated that the method has applicability and robustness for use in biodistribution studies.

金属酞菁是光动力疗法中有希望的成分。氯化铝酞菁铝（AlClPc）已用于治疗小鼠，人类龋齿组织，肺癌细胞和其他疾病的口腔癌。为了克服AlClPc的高疏水性，通常将酞菁封装在纳米制剂中。尽管使用量增加，但是关于AlClPc的药代动力学和生物分布知之甚少。这项研究的目的是开发和验证一种从纳米制剂和生物基质（例如血浆和组织）中提取后测定溶液中AlClPc的UHPLC-MS方法。已经描述了所描述的方法的选择性，线性，检测和定量限，精密度和回收率。本研究是第一个通过质谱法描述AlClPc在生物基质中的行为，也是第一个描述AlClPc污染物的色谱行为。分子质量分析确定了LC / MS和MALDI-MS对AlClPc的脱氯作用以及LC / MS中加合物的形成。观察到的参数表明该方法在生物分布研究中具有适用性和鲁棒性。