The application of electrophoresis upon commercial threads is investigated for development of low-cost diagnostics assays, designed for the matrix separation and quantification of low abundance metabolites in complex samples - in this work riboflavin in human urine. Zone electrophoresis was evaluated upon 8 commercially available threads, with several synthetic threads exhibiting higher electroosmotic flow (EOF) and increased electrophoretic mobility of the rhodamine 6G, rhodamine B, and fluorescein. Of those tested, a nylon bundle was selected as the best platform, offering less band dispersion and higher resolution, a high relative EOF, whilst minimising the contribution of joule heating. A novel 3D printed platform was designed, based on a modular system, facilitating the electrophoresis process and rapid assembly, whilst offering the potential for multiplexed analysis or investigation of more complex systems. Using the thread-based electrophoresis system, riboflavin was determined in less than 2 min. The device exhibited a linear working range from 0.1 to 15 μg/mL of riboflavin in urine, and was in good agreement with capillary electrophoresis measurements.

中文翻译：

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用于复杂样品中直接代谢物分析的基于线程的电流体平台

研究了电泳在商业线程上的应用，以开发低成本诊断分析，设计用于复杂样品中低丰度代谢物的基质分离和定量 - 在这项工作中，人类尿液中的核黄素。对 8 种市售螺纹进行了区带电泳评估，其中几种合成螺纹表现出更高的电渗流 (EOF) 和增加的罗丹明 6G、罗丹明 B 和荧光素的电泳迁移率。在这些测试中，尼龙束被选为最佳平台，提供更少的谱带色散和更高的分辨率、高相对 EOF，同时最大限度地减少焦耳热的贡献。基于模块化系统设计了一种新颖的 3D 打印平台，便于电泳过程和快速组装，同时提供了对更复杂系统进行多重分析或调查的潜力。使用基于螺纹的电泳系统，核黄素的测定时间不到 2 分钟。该装置在尿液中显示出 0.1 至 15 μg/mL 的线性工作范围内的核黄素，并且与毛细管电泳测量结果非常一致。