Background

The mechanisms that regulate maintenance of persistent T_H2 cells and potentiate allergic inflammation are not well understood.

Objective

The function of serine protease inhibitor 2A (Spi2A) was studied in mouse T_H2 cells, and the serine protease inhibitor B3 (SERPINB3) and SERPINB4 genes were studied in T_H2 cells from patients with grass pollen allergy.

Methods

Spi2A-deficient T_H2 cells were studied in in vitro culture or in vivo after challenge of Spi2A knockout mice with ovalbumin in alum. Expression of SERPINB3 and SERPINB4 mRNA was measured in in vitro–cultured T_H2 cells and in ex vivo CD27⁻CD4⁺ cells and innate lymphoid cell (ILC) 2 from patients with grass pollen allergy by using quantitative PCR. SERPINB3 and SERPINB4 mRNA levels were knocked down in cultured CD27⁻CD4⁺ cells with small hairpin RNA.

Results

There were lower levels of in vitro–polarized T_H2 cells from Spi2A knockout mice (P < .005) and in vivo after ovalbumin challenge (P < .05), higher levels of apoptosis (Annexin V positivity, P < .005), and less lung allergic inflammation (number of lung eosinophils, P < .005). In vitro–polarized T_H2 cells from patients with grass pollen allergy expressed higher levels of both SERPINB3 and SERPINB4 mRNA (both P < .05) compared with unpolarized CD4 T cells. CD27⁻CD4⁺ from patients with grass pollen allergy expressed higher levels of both SERPINB3 and SERPINB4 mRNA (both P < .0005) compared with CD27⁺CD4⁺ cells. ILC2 expressed higher levels of both SERPINB3 and SERPINB4 mRNA (both P < .0005) compared with ILC1. Knockdown of either SERPINB3 or SERPINB4 mRNA (both P < .005) levels resulted in decreased viability of CD27⁻CD4⁺ compared with control transduced cells.

Conclusion

The Serpins Spi2A in mice and SERPINB3 and SERPINB4 in allergic patients control the viability of T_H2 cells. This provides proof of principle for a therapeutic approach for allergic disease through ablation of allergic memory T_H2 cells through SERPINB3 and SERPINB4 mRNA downregulation.

中文翻译：

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抗凋亡丝氨酸蛋白酶抑制剂有助于过敏性 TH2 细胞的存活

 背景

调节持续性 T _H 2 细胞的维持和增强过敏性炎症的机制尚不清楚。

 客观的

在小鼠 T _H 2 细胞中研究了丝氨酸蛋白酶抑制剂 2A (Spi2A) 的功能，在草花粉过敏患者的 T _H 2 细胞中研究了丝氨酸蛋白酶抑制剂 B3 (SERPINB3)和SERPINB4基因。

 方法

在用明矾中的卵清蛋白攻击 Spi2A 敲除小鼠后，在体外培养或体内研究了 Spi2A 缺陷的 T _H 2 细胞。使用定量 PCR 测量体外培养的 T _H 2 细胞和体外培养的草花粉过敏患者的 CD27 ^- CD4 ⁺细胞和先天淋巴细胞 (ILC) 2 中SERPINB3和SERPINB4 mRNA 的表达。在具有小发夹 RNA 的培养 CD27 ^- CD4 ⁺细胞中， SERPINB3和SERPINB4 mRNA 水平被敲低。

 结果

Spi2A 敲除小鼠的体外极化 T _H 2 细胞水平较低 ( P < .005)，卵清蛋白攻击后的体内极化 T H 2 细胞水平较低 ( P < .05)，细胞凋亡水平较高（Annexin V 阳性， P < .005），肺部过敏性炎症较少（肺嗜酸性粒细胞数量， P < .005）。与未极化的 CD4 T 细胞相比，来自草花粉过敏患者的体外极化 T _H 2 细胞表达更高水平的SERPINB3和SERPINB4 mRNA（均为P < .05）。与 CD27 ⁺ CD4 ⁺细胞相比，草花粉过敏患者的 CD27 ^- CD4 ⁺表达更高水平的SERPINB3和SERPINB4 mRNA（均为P < .0005）。与 ILC1 相比，ILC2 表达更高水平的SERPINB3和SERPINB4 mRNA（均为P < .0005）。与对照转导细胞相比， SERPINB3或SERPINB4 mRNA（均为P < .005）水平的敲低导致 CD27 ^- CD4 ⁺的活力降低。

 结论

小鼠中的丝氨酸蛋白酶抑制剂 Spi2A 以及过敏患者中的 SERPINB3 和 SERPINB4 控制 T _H 2 细胞的活力。这为通过SERPINB3和SERPINB4 mRNA 下调消除过敏性记忆 T _H 2 细胞来治疗过敏性疾病提供了原理证明。