Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal amplification technique, operating at 37–42°C, with minimal sample preparation and capable of amplifying as low as 1–10 DNA target copies in less than 20 min. It has been used to amplify diverse targets, including RNA, miRNA, ssDNA and dsDNA from a wide variety of organisms and samples. An ever increasing number of publications detailing the use of RPA are appearing and amplification has been carried out in solution phase, solid phase as well as in a bridge amplification format. Furthermore, RPA has been successfully integrated with different detection strategies, from end-point lateral flow strips to real-time fluorescent detection amongst others. This review focuses on the different methodologies and advances related to RPA technology, as well as highlighting some of the advantages and drawbacks of the technique.

重组酶聚合酶扩增（RPA）是一种高度敏感的选择性等温扩增技术，在37–42°C的温度下操作，样品制备量最少，并且能够在不到20分钟的时间内扩增低至1–10个DNA目标拷贝。它已被用于扩增多种靶标，包括来自多种生物和样品的RNA，miRNA，ssDNA和dsDNA。越来越多的出版物详细介绍了RPA的使用，并且已经在溶液相，固相以及桥式扩增形式中进行了扩增。此外，RPA已成功地与不同的检测策略集成在一起，从端点横向流动条到实时荧光检测等。这篇评论着重于与RPA技术相关的不同方法和进展，