Thermoresponsive surfaces, prepared by grafting of poly(N-isopropylacrylamide) (PIPAAm) or its copolymers, have been investigated for biomedical applications. Thermoresponsive cell culture dishes that show controlled cell adhesion and detachment following external temperature changes, represent a promising application of thermoresponsive surfaces. These dishes can be used to fabricate cell sheets, which are currently used as effective therapies for patients. Thermoresponsive microcarriers for large-scale cell cultivation have also been developed by taking advantage of the thermally modulated cell adhesion and detachment properties of thermoresponsive surfaces. Furthermore, thermoresponsive bioseparation systems using thermoresponsive surfaces for separating and purifying pharmaceutical proteins and therapeutic cells have been developed, with the separation systems able to maintain their activity and biological potency throughout the procedure. These applications of thermoresponsive surfaces have been improved with progress in preparation techniques of thermoresponsive surfaces, such as polymerization methods, and surface modification techniques. In the present review, the various types of PIPAAm-based thermoresponsive surfaces are summarized by describing their preparation methods, properties, and successful biomedical applications.

中文翻译：

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基于聚（N-异丙基丙烯酰胺）的热敏表面提供了新型的生物医学应用

通过接枝聚（N-异丙基丙烯酰胺（PIPAAm）或其共聚物已被研究用于生物医学应用。在外部温度变化后表现出受控的细胞粘附和脱离的热响应性细胞培养皿代表了热响应性表面的有希望的应用。这些培养皿可用于制造细胞片，细胞片目前被用作患者的有效疗法。通过利用热响应性表面的热调节细胞粘附和分离特性，还开发了用于大规模细胞培养的热响应性微载体。此外，已开发出使用热响应表面分离和纯化药物蛋白和治疗性细胞的热响应生物分离系统，分离系统能够在整个过程中保持其活性和生物效力。随着诸如聚合方法和表面改性技术之类的热响应性表面的制备技术的进步，热响应性表面的这些应用得到了改善。在本综述中，通过描述它们的制备方法，性质和成功的生物医学应用，总结了各种类型的基于PIPAAm的热响应性表面。