A sensitive and reliable technique for meat species identification is required to prevent food adulteration, particularly in meat production. This work developed an optimized multiplex PCR assay for simultaneous identification of five commonly consumed and five commonly banned meat species in meat products. We designed primers that specifically amplified mitochondrial ATPase subunit 8 gene regions of different lengths of bovine, ovine, swine, chicken, turkey, cat, dog, mouse and human DNAs. The developed multiplex PCR assay proved to be specific, sensitive down to 30 pg DNA per reaction, reproducible and economical. It could be used with a variety of raw meats and processed food samples and is easily applicable in a routine laboratory analysis without specific sophisticated equipment.

需要一种灵敏可靠的肉类鉴定技术来防止食品掺假，特别是在肉类生产中。这项工作开发了一种优化的多重PCR分析方法，可同时识别肉类产品中的五种常用食用肉类和五种常用禁用肉类。我们设计了特异性扩增不同长度的牛，绵羊，猪，鸡，火鸡，猫，狗，小鼠和人类DNA的线粒体ATPase亚基8个基因区域的引物。事实证明，开发的多重PCR检测具有特异性，每个反应灵敏度低至30 pg DNA，可重复且经济。它可以与各种生肉和加工食品样品一起使用，无需特殊的精密设备即可轻松应用于常规实验室分析。