In the present study, a facile fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks of N,N-bis(2-hydroxyethyl)dithiooxamidato copper(II) (H2dtoaCu) was developed for the sensitive detection of adenosine triphosphate (ATP). The sensing mechanism was based on the noncovalent interaction between FAM-labeled (fluorescein amidite) ATP aptamers and H2dtoaCu. In the absence of ATP, the FAM-labeled aptamer readily adsorbs onto H2dtoaCu, mainly via π-π stacking and hydrogen bond interactions between the nucleotide bases and the H2dtoaCu surface, leading to the reduction of fluorescence intensity of the FAM by photoinduced electron transfer (PET). In the presence of ATP, the FAM-labeled aptamer specifically forms ATP-binding aptamer complexes which exhibit only weak adsorption on the H2dtoaCu surface. Thus, the fluorescence of the FAM-labeled ATP aptamer remained largely unchanged. The fluorescence aptasensor exhibited a good linear relationship between the fluorescence intensity and the logarithm concentration of ATP over a range of 25-400 nM, with a detection limit of 8.19 nM (3S/N). ATP analogs such as guanosine triphosphate, uridine triphosphate, and cytidine triphosphate have negligible effect on the aptasensor performance due to the high selectivity of the ATP aptamer to its target, showing promising potential in real sample analysis.

中文翻译：

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基于二维金属片-有机骨架的荧光适体传感器监测三磷酸腺苷

在本研究中，开发了一种基于 N,N-双（2-羟乙基）二硫代胺基铜 (II) (H2dtoaCu) 二维金属片有机骨架的简易荧光适体传感器，用于三磷酸腺苷 (ATP) 的灵敏检测. 传感机制基于 FAM 标记的（荧光素亚酰胺）ATP 适体和 H2dtoaCu 之间的非共价相互作用。在没有 ATP 的情况下，FAM 标记的适体很容易吸附到 H2dtoaCu 上，主要是通过 π-π 堆积和核苷酸碱基与 H2dtoaCu 表面之间的氢键相互作用，通过光诱导电子转移导致 FAM 的荧光强度降低。宠物）。在 ATP 存在下，FAM 标记的适体特异性地形成 ATP 结合适体复合物，其在 H2dtoaCu 表面上仅表现出弱吸附。因此，FAM 标记的 ATP 适体的荧光基本保持不变。该荧光适体传感器在25-400 nM范围内，荧光强度与ATP对数浓度呈良好的线性关系，检测限为8.19 nM（3S/N）。由于 ATP 适体对其靶标具有高选择性，因此三磷酸鸟苷、三磷酸尿苷和三磷酸胞苷等 ATP 类似物对适体传感器性能的影响可以忽略不计，在实际样品分析中显示出巨大的潜力。